Cryo electron tomography of Cytochalasin D-induced protrusions of Drosophila S2 cells treated with DMSO or thapsigargin - Datasets 5

REL 2023-02-17 2023-07-10 2023-07-10 Cryo electron tomography of Cytochalasin D-induced protrusions of Drosophila S2 cells treated with DMSO or thapsigargin - Datasets 5 - 7 0000-0002-4406-5151 Camilla Ventura Santos MRC Laboratory of Molecular Biology Cambridge Biomedical Campus, Francis Crick Ave Cambridge Cambridgeshire United Kingdom CB2 0QH 0000-0001-7292-5430 Andrew Carter MRC Laboratory of Molecular Biology Cambridge Biomedical Campus, Francis Crick Ave Cambridge Cambridgeshire United Kingdom CB2 0QH Ventura Santos C Carter AP Rogers SL Medical Research Council (MRC, United Kingdom) MC_UP_A025_1011 United Kingdom Welch Foundation 210711/Z/18/Z United Kingdom 373.1 10.6019/EMPIAR-11451 EMDB cell EMD-16720 Ventura Santos C Carter AP Rogers SL CryoET shows cofilactin filaments inside the microtubule lumen EMPIAR-11450 Dataset 5: Raw multi-frame tilt images of Cytochalasin D and DMSO or thapsigarging treated Drosophila S2 cells /data/Dataset5_220617/raw_movies micrographs - multiframe TIFF TIFF 24 14 UNSIGNED 16 BIT INTEGER 3710 2.952 3838 2.952

Data from Dataset 5. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours and treated with either 0.1% DMSO (TS_201 - TS_214) or 2 uM thapsigargin (TS_215 - TS_228). The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) can be found within the Mdoc_files folder.

Dataset 5: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions /data/Dataset5_220617/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 24 14 32 BIT FLOAT 960 11.81 928 11.81

Dataset 5: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP. Tomograms TS_201 - TS_214 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 0.1% DMSO (5 hours). TS_215 - TS_228 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 2 uM thapsigargin (5 hours).

Dataset 6: Raw multi-frame tilt images of Cytochalasin D and DMSO or thapsigarging treated Drosophila S2 cells /data/Dataset6_220720/raw_movies micrographs - multiframe TIFF TIFF 33 14 UNSIGNED 16 BIT INTEGER 3710 2.952 3838 2.952

Data from Dataset 6. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours and treated with either 0.1% DMSO (TS_262 - TS_284) or 2 uM thapsigargin (TS_153 - TS_161 and TS_243 - TS_251). The gain reference and defects files are provided within the folder. The pixel size is 2.952 Å/pixel. Data was collected on a K2 summit. Metadata from tilt series acquisition (Mdoc files) can be found within the Mdoc_files folder.

Dataset 6: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions /data/Dataset6_220720/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 33 14 32 BIT FLOAT 960 11.81 928 11.81

Dataset 6: Tomograms binned by 4 (11.808 Å/pixel), reconstructed and deconvolved in WARP. Tomograms TS_262 - TS_282 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 0.1% DMSO (5 hours). TS_153 - TS_161 and TS_243 - TS_251 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 2 uM thapsigargin (5 hours).

Dataset 7: Raw multi-frame tilt images of Cytochalasin D and DMSO or thapsigarging treated Drosophila S2 cells /data/Dataset7_220721/raw_movies micrographs - multiframe TIFF TIFF 33 14 UNSIGNED BYTE 5760 2.659 4092 2.659

Data from Dataset 7. Raw multi-frame movies of tilt series collected on protrusions of Drosophila S2 cells induced with 2.5 uM Cytochalasin D for 4 hours and treated with either 0.1% DMSO (TS_304 - TS_328) or 2 uM thapsigargin (TS_285 - TS_303 and TS_329). The gain reference is provided within the folder. The pixel size is 2.659 Å/pixel. Data was collected on a K3 detector. Metadata from tilt series acquisition (Mdoc files) can be found within the Mdoc_files folder.

Dataset 7: Binned, deconvolved tomograms of induced Drosophila S2 cell protrusions /data/Dataset7_220721/bin4_deconvolved_tomograms reconstructed volumes MRC MRC 33 14 32 BIT FLOAT 1022 10.64 1440 10.64

Dataset 6: Tomograms binned by 4 (10.64 Å/pixel), reconstructed and deconvolved in WARP. Tomograms TS_304 - TS_328 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 0.1% DMSO (5 hours). TS_285 - TS_303 and TS_329 are of cells treated with 2.5 uM Cytochalasin D (4 hours) and 2 uM thapsigargin (5 hours).