REL
2023-03-10
2023-04-26
2023-05-16
Cryo-electron tomography of ChAdOx spikes (AZD2816)
0000-0001-7268-0306
Tao
Ni
The University of Hong Kong
Hong Kong
Hong Kong
0000
0000-0003-1803-691X
Peijun
Zhang
The University of Oxford
Oxford
United Kingdom
OX3 7BN
Ni T
Mendonca L
Zhu Y
Howe A
Radecke J
Sheng Y
Krebs AS
Shah P
Allen E
Spencer A
Morris S
Stuart D
Gilbert S
Zhang P
The tilt-series have been motion corrected. The transformation files, tilt angle and gold beads positions are generated from Etomo. Tilt-series were acquired using ThermoFisher Titan Krios microscopes operated at 300 keV, equipped with a K3 camera and Quantum energy filter in zeros-loss mode. The energy slit width was set to 20 eV. The tilt-series were collected with serialEM using a dose-symmetric scheme, starting from 0° with a 3° tilt increment by a group of 3, with an angular range from -60° to 60°. The accumulated exposure for each tilt-series was ~120 e-/Å2, with a defocus range between -2 and -5 µm.
Cryo-electron tomography of ChAdOx spikes 2Pro mutant in situ
/data/tilt-series
tilt series
MRC
MRC
142
41
35
41
32 BIT FLOAT
5760
2.18
4092
2.18
Tilt-series were acquired using ThermoFisher Titan Krios microscopes operated at 300 keV, equipped with a K3 camera and Quantum energy filter in zeros-loss mode. The energy slit width was set to 20 eV. The tilt-series were collected with serialEM using a dose-symmetric scheme, starting from 0° with a 3° tilt increment by a group of 3, with an angular range from -60° to 60°. The accumulated exposure for each tilt-series was ~120 e-/Å2, with a defocus range between -2 and -5 µm. The micrographs have been motion-corrected and stacked using IMOD newstack command. The dataset contains the motion-corrected tilt-series, transformation matrix file (*.xf), local transformation file (*local), tilt angle (*tlt) and gold beads positions (*erase), which are generated from IMOD.