Single particle cryo-EM dataset of Mus musculus mitochondrial complex I bound with inhibitor Piericidin A

REL 2023-04-24 2023-08-11 2023-08-11 Single particle cryo-EM dataset of Mus musculus mitochondrial complex I bound with inhibitor Piericidin A 0000-0001-8667-6797 Judy Hirst MRC mitochondrial Biology Unit, University of Cambridge The Keith Peters Building, Cambridge Biomedical Campus, Hills Road Cambridge Cambridgeshire United Kingdom CB2 0XY 0000-0001-8667-6797 Judy Hirst MRC mitochondrial Biology Unit, University of Cambridge The Keith Peters Building, Cambridge Biomedical Campus, Hills Road Cambridge Cambridgeshire United Kingdom CB2 0XY Grba DN Chung I Bridges HR Agip AA Hirst J Medical Research Council (MRC, United Kingdom) MC_UU_00015/2 United Kingdom Medical Research Council (MRC, United Kingdom) MC_UU_00028/1 United Kingdom 1.9 10.6019/EMPIAR-11531 EMDB molecule EMD-16962 Grba DN Chung I Bridges HR Agip AA Hirst J Investigation of hydrated channels and proton pathways in a high-resolution cryo-EM structure of mammalian complex I Science advances Sci Adv 31 9 2023 English 10.1126/sciadv.adi1359 37531432 Unaligned multi-frame micrographs of wild-type mouse mitochondrial complex I from an as-prepared inhibited by piericidin A /data micrographs - multiframe MRC MRC 1565 40 1 40 SIGNED 16 BIT INTEGER 4096 1.06 4096 1.06

Gain-corrected micrographs were imaged using a Falcon III detector in counting mode on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. The sample was frozen on PEGylated gold grids.