REL 2023-09-25 2023-09-25 2023-09-25 0000-0002-1941-5535 Hideaki Kato Komaba Institute for Science, The University of Tokyo 4-6-1 Komaba Meguro Tokyo Japan 153-8505 0000-0001-9440-3967 Karl Deisseroth Department of Bioenginerring, Stanford Univerisity 1291 Welch Road Clark Center Basement Rm W080 Stanford California United States 94305 Hideaki Kato Komaba Institute for Science, The University of Tokyo Meguro Japan 153-8505 Tajima S Kim YS Fukuda M Nakamura S Yamashita K Deisseroth K 4.1 10.6019/EMPIAR-11558 EMDB molecule EMD-34530 EMD-34531 EMD-35713 Tajima S Kim YS Fukuda M Jo Y Wang PY Paggi JM Inoue M Byrne EFX Kishi KE Nakamura S Ramakrishnan C Takaramoto S Nagata T Konno M Sugiura M Katayama K Matsui TE Yamashita K Kim S Ikeda H Kim J Kandori H Dror RO Inoue K Deisseroth K Kato HE Cell Cell 2023 English 10.1016/j.cell.2023.08.009 37652010 HcKCR1 WT in lipid nanodisc /data/HcKCR1WT micrographs - multiframe TIFF TIFF 5445 48 1 48 UNSIGNED BYTE 5760 0.83 4092 0.83

Please use CountRef_tajima4_2_ND16_116-1_0000_Nov26_12.53.53.mrc as a gain reference without rotation or flipping. Please ignore the pixel size in the header (0.85 Å/px); use 0.83 Å/px. 300 kV Cs 2.7 mm 0.996 e/A2/frame

HcKCR1 H225F in lipid nanodisc /data/HcKCR1_H225F micrographs - multiframe TIFF TIFF 6654 48 1 48 UNSIGNED BYTE 5760 0.83 4092 0.83

Please use gain_reference/movie_gain.mrc as a gain reference without rotation or flipping. Please ignore the pixel size in the header (1.00 Å/px); use 0.83 Å/px. 300 kV Cs 2.7 mm 0.991 e/A2/frame

HcKCR2 WT in lipid nanodisc /data/HcKCR2WT micrographs - multiframe TIFF TIFF 7718 48 1 48 UNSIGNED BYTE 5760 0.83 4092 0.83

Please use gain_reference/movie_gain.mrc as a gain reference without rotation or flipping. Please ignore the pixel size in the header (1.00 Å/px); use 0.83 Å/px. 300 kV Cs 2.7 mm 1.06 e/A2/frame