REL
2023-05-03
2023-09-22
2023-09-22
Carboxysome minishell containing CsoS1A4A and truncated CsoS2
0000-0001-7268-0306
Tao
Ni
The University of Hong Kong
Hong Kong
Hong Kong
000000
0000-0003-1803-691X
Peijun
Zhang
The University of Oxford
Oxford
United Kingdom
OX3 7BN
Ni T
Jiang Q
Ng PC
Dou H
Liu LN
Zhang P
Wellcome Trust
206422/Z/17/Z
United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)
BBSRC: BB/S003339/1, BB/V009729/1 and BB/R003890/1
United Kingdom
National Natural Science Foundation of China (NSFC)
National Natural Science Foundation of China (32070109)
China
European Research Council (ERC)
ERC AdG grant (101021133)
United Kingdom
Royal Society
Royal Society (URF\R\180030, RGF\EA\181061 and RGF\EA\180233),
United Kingdom
2.2
10.6019/EMPIAR-11560
EMDB
molecule
EMD-15722
EMD-15720
EMD-15595
EMD-15723
EMD-15724
EMD-15719
Ni T
Jiang Q
Ng PC
Shen J
Dou H
Zhu Y
Radecke J
Dykes GF
Huang F
Liu LN
Zhang P
Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly
Nature communications
Nat Commun
1
14
2023
English
10.1038/s41467-023-41211-y
37679318
The mini-shell-4 samples which is obtained by co-expression of CsoS1A, CsoS4A and CosS2 C1 mutant are imaged.The cryoEM sample grids were prepared using Vitrobot. Three microliters of mini-shell samples were applied to glow-discharged Quantifoil grids (R2/1) and blotted with filter paper for 3 seconds before plunge freezing with liquid nitrogen-cooled ethane. The temperature was set to 20 ℃ and humidity at 100% during plunge freezing. The micrographs were taken using Krios microscope equipped with a Gatan K3 director electron camera and BioQuantum energy filter (slit width 20 eV). Total exposure dose is 44 electrons/angstrom2, fractionated into 50 frames in tif format. The raw movies are not gain normalised.
Unaligned raw frames of mini shell co-expressing CsoS1A, CsoS4A and truncated CsoS2 (C1 construct)
/data
micrographs - multiframe
TIFF
TIFF
13151
40
UNSIGNED BYTE
5760
0.831
4092
0.831
The micrographs are raw data frames with 40 fractions, collected with Gatan K3 camera in super-resolution but 2x binned in EPU. The gain reference is in the same dimension as the 2x binned micrographs.