REL
2023-05-31
2024-03-18
2024-03-18
Seeded Aβ(1-40) amyloid fibril
0000-0002-9442-460X
Matthias
Schmidt
Institut für Proteinbiochemie, Universität Ulm
8/1 Helmholtzstrasse
Ulm
Baden Württemberg
Germany
89081
0000-0003-2123-6816
Marcus
Fändrich
Institut für Proteinbiochemie, Universität Ulm
8/1 Helmholtzstrasse
Ulm
Baden Württemberg
Germany
89081
Pfeiffer PB
Schmidt M
Fändrich M
German Research Foundation (DFG)
FA 456/24-1
Germany
550.2
10.6019/EMPIAR-11596
EMDB
molecule
EMD-17168
Pfeiffer PB
Ugrina M
Schwierz N
Sigurdson CJ
Schmidt M
Fändrich M
Cryo-EM analysis of the effect of seeding with brain-derived Aβ amyloid fibrils
Germany
English
Unaligned multi-frame micrographs of seeded Aβ(1-40) amyloid fibrils
/data/data
micrographs - multiframe
TIFF
TIFF
3018
40
UNSIGNED 16 BIT INTEGER
3838
1.04
3710
1.04
Krios1 Quantum-K2
Cs 2.7
300kV EFTEM nanpprobe
x130k spot 8 beam size 650nm
c2=50 objective_aperture=100
GIF-K2 counting
10s exposure 40 frames
total dose at the sample 53.79e/A2
1 pixel = 1.04A
dose rate ~4-5 e/pix/s
defocus range -0.8 to -2.0
The gain reference (/data/data/CountRef_map_0117_001.dm4) is not applied to the micrographs. The NOTES.txt file contains EM information. Thumbnails and power spectra (/data/thumbs) as well as coordinates (/data/manual pick) are provided.
data/data/*.tif
data/manual pick/*.star
data/manual pick