<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11648" schemaVersion="0.63" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2023-08-02</depositionDate>
            <releaseDate>2023-10-03</releaseDate>
            <updateDate>2023-10-03</updateDate>
        </keyDates>
        <title>Single particle cryo-EM dataset of mouse mitochondrial complex I in the active state</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-6890-6050</authorORCID>
            <firstName>Hannah</firstName>
            <middleName>Ruth</middleName>
            <lastName>Bridges</lastName>
            <organization type="academic">MRC Mitochondrial Biology Unit</organization>
            <street>Hills Road</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB2 0XY</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-8667-6797</authorORCID>
            <firstName>Judy</firstName>
            <lastName>Hirst</lastName>
            <organization type="academic">MRC Mitochondrial Biology Unit</organization>
            <street>Hills Road</street>
            <townOrCity>Cambridge</townOrCity>
            <stateOrProvince>Cambridgeshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>CB2 0XY</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-3020-8262">Agip AA</author>
            <author authorORCID="0000-0001-5420-2116">Blaza JN</author>
            <author authorORCID="0000-0001-6890-6050">Bridges HR</author>
            <author authorORCID="0000-0001-8667-6797">Hirst J</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Medical Research Council (MRC, United Kingdom)</fundingBody>
                <code>MC_U105663141</code>
                <country>United Kingdom</country>
            </grantReference>
            <grantReference>
                <fundingBody>Medical Research Council (MRC, United Kingdom)</fundingBody>
                <code>MC_UU_00015/2</code>
                <country>United Kingdom</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">1.6</datasetSize>
        <entryDOI>10.6019/EMPIAR-11648</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-4345</emdbEntry>
            <emdbEntry>EMD-11377</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-3020-8262" order="1">Agip AA</author>
                    <author authorORCID="0000-0001-5420-2116" order="2">Blaza JN</author>
                    <author authorORCID="0000-0001-6890-6050" order="3">Bridges HR</author>
                    <author authorORCID="0000-0001-6050-0566" order="4">Viscomi C</author>
                    <author authorORCID="0000-0002-2973-2630" order="5">Rawson S</author>
                    <author authorORCID="0000-0001-6869-4414" order="6">Muench SP</author>
                    <author authorORCID="0000-0001-8667-6797" order="7">Hirst J</author>
                    <title>Cryo-EM structures of complex I from mouse heart mitochondria in two biochemically defined states</title>
                    <journal>Nature structural &amp; molecular biology</journal>
                    <journalAbbreviation>Nat Struct Mol Biol</journalAbbreviation>
                    <country></country>
                    <issue>7</issue>
                    <volume>25</volume>
                    <firstPage>548</firstPage>
                    <lastPage>556</lastPage>
                    <year>2018</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41594-018-0073-1</externalReferences>
                    <externalReferences type="pubmed">29915388</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned, gain-corrected micrographs of mitochondrial complex I from mouse heart</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>1235</numImagesOrTiltSeries>
        <framesPerImage>25</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>25</frameRangeMax>
        </frameRange>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>3838</imageWidth>
            <pixelWidth>1.054</pixelWidth>
            <imageHeight>3710</imageHeight>
            <pixelHeight>1.054</pixelHeight>
        </dimensions>
        <details>The micrographs are the average of 25 unaligned, gain-corrected frames.The dose rate was 5 electrons Å-2 s-1 with 10 s exposure, giving a total dose of 50 e Å-2. The data were recorded on a Krios using a K2 and energy filter (slit width 20 eV). C2 aperture of 70 μm and Objective aperture of 100 μm and defocus range of -1.5 to -3.1 μm.</details>
        <segmentationList/>
        <micrographsFilePattern>data/*.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
