<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11734" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2023-09-20</depositionDate>
            <releaseDate>2023-10-31</releaseDate>
            <updateDate>2024-04-17</updateDate>
        </keyDates>
        <title>Structure and dynamics of a pentameric KCTD5/Cullin3/GBeta1Gamma2 E3 ubiquitin ligase complex</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-3584-4804</authorORCID>
            <firstName>Douglas</firstName>
            <middleName>A</middleName>
            <lastName>Kuntz</lastName>
            <organization type="academic">Princess Margaret Cancer Centre</organization>
            <street>101 College St</street>
            <townOrCity>Toronto</townOrCity>
            <stateOrProvince>Ontario</stateOrProvince>
            <country>Canada</country>
            <postOrZipCode>M6G 1L7</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-0712-4319</authorORCID>
            <firstName>Gilbert</firstName>
            <middleName>G</middleName>
            <lastName>Prive</lastName>
            <organization type="academic">Princess Margaret Cancer Centre</organization>
            <street>101 College St</street>
            <townOrCity>Toronto</townOrCity>
            <stateOrProvince>Ontario</stateOrProvince>
            <country>Canada</country>
            <postOrZipCode>M5G 1L7</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-8646-7976">Nguyen DM</author>
            <author authorORCID="0000-0001-8327-1559">Narayanan N</author>
            <author authorORCID="0000-0003-3584-4804">Kuntz DA</author>
            <author authorORCID="0000-0002-0712-4319">Prive GG</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Canadian Institutes of Health Research (CIHR)</fundingBody>
                <code>PJT162186</code>
                <country>Canada</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">8.5</datasetSize>
        <entryDOI>10.6019/EMPIAR-11734</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-41994</emdbEntry>
            <emdbEntry>EMD-41995</emdbEntry>
            <emdbEntry>EMD-41996</emdbEntry>
            <emdbEntry>EMD-41997</emdbEntry>
            <emdbEntry>EMD-41998</emdbEntry>
            <emdbEntry>EMD-41999</emdbEntry>
            <emdbEntry>EMD-42000</emdbEntry>
            <emdbEntry>EMD-42001</emdbEntry>
            <emdbEntry>EMD-42002</emdbEntry>
            <emdbEntry>EMD-42003</emdbEntry>
            <emdbEntry>EMD-42004</emdbEntry>
            <emdbEntry>EMD-42005</emdbEntry>
            <emdbEntry>EMD-42006</emdbEntry>
            <emdbEntry>EMD-42007</emdbEntry>
            <emdbEntry>EMD-42008</emdbEntry>
            <emdbEntry>EMD-42009</emdbEntry>
            <emdbEntry>EMD-42010</emdbEntry>
            <emdbEntry>EMD-42011</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-8646-7976" order="1">Nguyen DM</author>
                    <author authorORCID="0000-0003-3127-1711" order="2">Rath DH</author>
                    <author authorORCID="0000-0001-5278-1062" order="3">Devost D</author>
                    <author order="4">Pétrin D</author>
                    <author order="5">Rizk R</author>
                    <author order="6">Ji AX</author>
                    <author order="7">Narayanan N</author>
                    <author authorORCID="0009-0000-6487-7467" order="8">Yong D</author>
                    <author order="9">Zhai A</author>
                    <author authorORCID="0000-0003-3584-4804" order="10">Kuntz DA</author>
                    <author authorORCID="0009-0002-4795-5421" order="11">Mian MUQ</author>
                    <author authorORCID="0009-0009-1921-1709" order="12">Pomroy NC</author>
                    <author order="13">Keszei AFA</author>
                    <author order="14">Benlekbir S</author>
                    <author authorORCID="0000-0003-0207-5877" order="15">Mazhab-Jafari MT</author>
                    <author authorORCID="0000-0003-0566-2209" order="16">Rubinstein JL</author>
                    <author authorORCID="0000-0002-7079-653X" order="17">Hébert TE</author>
                    <author authorORCID="0000-0002-0712-4319" order="18">Privé GG</author>
                    <title>Structure and dynamics of a pentameric KCTD5/CUL3/Gβγ E3 ubiquitin ligase complex.</title>
                    <journal>Proceedings of the National Academy of Sciences of the United States of America</journal>
                    <journalAbbreviation>Proc Natl Acad Sci U S A</journalAbbreviation>
                    <country></country>
                    <issue>17</issue>
                    <volume>121</volume>
                    <year>2024</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1073/pnas.2315018121</externalReferences>
                    <externalReferences type="pubmed">38625940</externalReferences>
                    <details>bioRxiv preprint version posted September 20, 2023</details>
                </journalCitation>
            </universalCitation>
            <universalCitation>
                <journalCitation published="true" preprint="true">
                    <author authorORCID="0000-0002-8646-7976" order="1">Nguyen DM</author>
                    <author authorORCID="0000-0003-3127-1711" order="2">Rath DH</author>
                    <author authorORCID="0000-0001-5278-1062" order="3">Devost D</author>
                    <author authorORCID="0009-0008-7614-8905" order="4">Pétrin D</author>
                    <author order="5">Rizk R</author>
                    <author authorORCID="0009-0008-8086-3952" order="6">Ji AX</author>
                    <author authorORCID="0000-0001-8327-1559" order="7">Narayanan N</author>
                    <author authorORCID="0009-0000-6487-7467" order="8">Yong D</author>
                    <author authorORCID="0009-0002-3012-3055" order="9">Zhai A</author>
                    <author authorORCID="0000-0003-3584-4804" order="10">Kuntz DA</author>
                    <author authorORCID="0009-0002-4795-5421" order="11">Mian MU</author>
                    <author authorORCID="0009-0009-1921-1709" order="12">Pomroy NC</author>
                    <author authorORCID="0000-0002-8524-7998" order="13">Keszei AF</author>
                    <author authorORCID="0000-0001-5079-0547" order="14">Benlekbir S</author>
                    <author authorORCID="0000-0003-0207-5877" order="15">Mazhab-Jafari MT</author>
                    <author authorORCID="0000-0003-0566-2209" order="16">Rubinstein JL</author>
                    <author authorORCID="0000-0002-7079-653X" order="17">Hébert TE</author>
                    <author authorORCID="0000-0002-0712-4319" order="18">Privé GG</author>
                    <editor order="1"> </editor>
                    <title>Structure and dynamics of a pentameric KCTD5/Cullin3/Gβγ E3 ubiquitin ligase complex</title>
                    <journal>bioRxiv</journal>
                    <journalAbbreviation></journalAbbreviation>
                    <country></country>
                    <externalReferences type="doi">10.1101/2023.09.20.558662</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>K5C3Gbg1 Krios Data Grid 1 multiframe micrographs</name>
        <directory>/data/KriosData/20220624_K5C3Gbg_Grid1</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>432</numImagesOrTiltSeries>
        <framesPerImage>30</framesPerImage>
        <frameRange>
            <frameRangeMin>0</frameRangeMin>
            <frameRangeMax>29</frameRangeMax>
        </frameRange>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>1.03</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>1.03</pixelHeight>
        </dimensions>
        <details>Collected on Falcon 4i. Calibrated pixel size is 1.03 which differs from the header information. Folder also contains link information linking the file names to the downstream processed frames.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>K5C3Gbg1 Krios Data Grid 2 multiframe micrographs</name>
        <directory>/data/KriosData/20220624_K5C3Gbg_Grid2</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>4199</numImagesOrTiltSeries>
        <framesPerImage>30</framesPerImage>
        <frameRange>
            <frameRangeMin>0</frameRangeMin>
            <frameRangeMax>29</frameRangeMax>
        </frameRange>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>1.03</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>1.03</pixelHeight>
        </dimensions>
        <details>Collected on Falcon 4i. Calibrated pixel size is 1.03 which differs from the header information.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>K5C3Gbg Krios Data Grid 2 TILT multiframe micrographs. Collected with 40 degree tilt.</name>
        <directory>/data/KriosData/20220624_K5C3Gbg_Tilt40_Grid2</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>4039</numImagesOrTiltSeries>
        <framesPerImage>30</framesPerImage>
        <frameRange>
            <frameRangeMin>0</frameRangeMin>
            <frameRangeMax>29</frameRangeMax>
        </frameRange>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>1.03</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>1.03</pixelHeight>
        </dimensions>
        <details>Collected on Falcon 4i. Calibrated pixel size is 1.03 which differs from the header information. Collected with 40 degree tilt.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>MotionCorrected Data for normal(Grid1+Grid2) K5C3Gbg</name>
        <directory>/data/K5C3Gbg_motioncorrected</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>4367</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>1.03</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>1.03</pixelHeight>
        </dimensions>
        <details>Patch_aligned_doseweighted</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>MotionCorrected Data for K5C3Gbg Grid2 TILT</name>
        <directory>/data/K5C3Gbg_TILT_motioncorrected</directory>
        <category>micrographs - single frame</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>4039</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>1.03</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>1.03</pixelHeight>
        </dimensions>
        <details>Patch_aligned_doseweighted of the Tilted Data set</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>K5C3Gbg Particles</name>
        <directory>/data/K5C3Gbg_Particle_data/K5G3Gbg_particles</directory>
        <category>picked particles - single frame - processed</category>
        <headerFormat>MRCS</headerFormat>
        <dataFormat>MRCS</dataFormat>
        <numImagesOrTiltSeries>6918</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>32 BIT FLOAT</voxelType>
        <dimensions>
            <imageWidth>324</imageWidth>
            <pixelWidth>1.03</pixelWidth>
            <imageHeight>324</imageHeight>
            <pixelHeight>1.03</pixelHeight>
        </dimensions>
        <details>Particles picked from Patch aligned doseweighted  motion corrected images. Particles are from both the standard and tilted images.</details>
        <segmentationList/>
        <micrographsFilePattern>data/K5C3Gbg_motioncorrected/*_Fractions_patch_aligned_doseweighted.mrc</micrographsFilePattern>
        <pickedParticlesFilePattern>data/K5C3Gbg_Particle_data/K5G3Gbg_particles/*_Fractions_particles_local_aligned.mrcs</pickedParticlesFilePattern>
        <pickedParticlesDirectory>data/K5C3Gbg_Particle_data/Particles_for_StatesABCD</pickedParticlesDirectory>
    </imageSet>
</entry>
