REL 2023-08-03 2024-06-04 2024-06-04 0000-0002-9442-460X Matthias Schmidt Institut für Proteinbiochemie, Universität Ulm Helmholtzstrasse 8/1 Ulm Baden Württemberg Germany 89081 0000-0003-2123-6816 Marcus Fändrich Institut für Proteinbiochemie, Universität Ulm Helmholtzstrasse 8/1 Ulm Baden Württemberg Germany 89081 Sharma K Schmidt M Fändrich M 280.1 10.6019/EMPIAR-11745 EMDB molecule EMD-18715 EMD-18716 EMD-18717 Sharma K Stockert F Shenoy J Berbon M Abdul-Shukkoor MB Habenstein B Loquet A Schmidt M Fändrich M Nature communications Nat Commun 1 15 2024 English 10.1038/s41467-023-44489-0 38212334 Unaligned multi-frame micrographs of TDP-43 amyloid fibrils /data/EM_data micrographs - multiframe TIFF TIFF 1641 40 UNSIGNED 16 BIT INTEGER 3838 1.04 3710 1.04

The gain reference (/data/EM_data/CountRef_0001.dm4) is not applied to the raw micrographs. The NOTES.txt file contains EM information (copied below). The coordinates are divided into Morphology-1 (/data/Coordinatesfiles_Morphology1) and Morphology-2 (/data/Coordinatesfiles_Morphology2). --------------------------------------------------------------------------------------------------------------------------------------------------------------------------- NOTES.txt EMBL Heidelberg Krios1 Quantum-K2 Cs 2.7 300kV EFTEM nanpprobe x130k spot 8 beam size 650nm c2=50 objective_aperture=100 GIF-K2 counting 10s exposure 40 frames total dose at the sample 52.08e/A2 1 pixel = 1.04A dose rate ~3-4 e/pix/s defocus range -1.2 to -2.5

data/EM_data/*.tif data/Coordinatesfiles_Morphology*/*_manualpick.star data/Coordinatesfiles_Morphology*