REL
2024-01-25
2024-03-19
2024-11-19
Unveiling the ultrastructural landscape of extracellular matrix via lift-out cryo-FIBSEM and cryo-ET
0000-0003-4790-8078
Florian
K.M.
Schur
Institute of Science and Technology Austria
Am Campus 1
Klosterneuburg
Lower Austria
Austria
3400
0000-0003-4790-8078
Florian
K.M.
Schur
Institute of Science and Technology Austria
Am Campus 1
Klosterneuburg
Lower Austria
Austria
3400
Zens B.
Fäßler F.
Hansen J.M.
Hauschild R.
Datler J.
Hodirnau V.V.
Zheden V.
Alanko J.
Sixt M.
Schur F.K.M.
Austrian Science Fund
P33367
Austria
Austrian Science Fund
E435
Austria
European Research Council (ERC)
724373
Austria
Chan Zuckerberg Initiative
DAF2021-234754
United States
183.0
10.6019/EMPIAR-11897
EMDB
cell
EMD-18490
EMD-18491
EMD-18492
EMD-18493
EMD-18494
Zens B
Fäßler F
Hansen JM
Hauschild R
Datler J
Hodirnau VV
Zheden V
Alanko J
Sixt M
Schur FKM
Lift-out cryo-FIBSEM and cryo-ET reveal the ultrastructural landscape of extracellular matrix
The Journal of cell biology
J Cell Biol
6
223
2024
English
10.1083/jcb.202309125
38506714
42 tomograms were acquired on FIB milled lamellae from Cell-derived matrices (CDMs). Frames (including GainRef and defects file), .mdoc files for acquired tilt series, as well as IsoNet-filtered reconstructed tomograms are provided.
Frames
/data/TS_frames
micrographs - multiframe
MRC
TIFF
42
8
UNSIGNED BYTE
5760
2.137
4092
2.137
42 tilt series consisting of maximally 61 tilts each; each image consists of 8 frames. GainRef and defects file are included.
Raw tilt series
/data/TS_raw
tilt series
MRC
MRC
42
1
UNSIGNED 16 BIT INTEGER
5760
2.137
4092
2.137
42 tilt series; .mdoc files are included. Bad tilts were excluded from the tilt series (as described in the manuscript).
Isonet corrected tomograms
/data/isonet_corrected
reconstructed volumes
MRC
MRC
42
1
UNSIGNED 16 BIT INTEGER
510
17.1
720
17.1
Isonet-corrected tomograms, reconstructed from cleaned tilt series (as described in the manuscript).