REL
2024-02-14
2024-06-11
2024-06-11
Single particle cryo-EM of H. thermophilus chaperonin complex
0000-0001-8217-6242
Zengwei
Liao
The University of Tokyo
1-1-1 Yayoi, Bunkyo-ku
Tokyo
Tokyo
Japan
113-8657
0000-0003-1346-6435
Shinya
Fushinobu
The University of Tokyo
1-1-1 Yayoi, Bunkyo-ku
Tokyo
Tokyo
Japan
113-8657
Liao Z
Gopalasingam CC
Kameya M
Gerle C
Shigematsu H
Ishii M
Arakawa T
Fushinobu S
Japan Society for the Promotion of Science (JSPS)
23H00322
Japan
Japan Science and Technology
JPMJSP2108
Japan
1.3
10.6019/EMPIAR-11901
EMDB
molecule
EMD-37862
EMD-37863
Liao Z
Gopalasingam CC
Kameya M
Gerle C
Shigematsu H
Ishii M
Arakawa T
Fushinobu S
Liao Z
Fushinobu S
Structural insights into thermophilic chaperonin complexes
Structure (London, England : 1993)
Structure
2024
English
10.1016/j.str.2024.02.012
38492570
Unaligned multi-frame movies collected using serialEM on a CRYO ARM 300 EM operating at 300 kV equipped with an Omega-type in-column energy filter (slit width 20 eV) and a Gatan K3 electron detector (operated in correlated doubling sample mode). Movies were collected using the beam-image shift method (5×5×1 matrices), at a target defocus range of -1.2 to -1.6 µm and at a nominal magnification of ×60,000, corresponding to a calibrated pixel size of 0.752 Å/pixel. Each movie was recorded with an exposure time of 2.264 s, subdivided into 50 frames, with a total electron dose of 49.97 e-1 Å-2.
EMPIAR-11900
Unaligned multi-frame movies of H. thermophilus chaperonin complex
/data
micrographs - multiframe
TIFF
TIFF
5650
50
0
50
UNSIGNED BYTE
5760
0.752
4092
0.752