<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-11946" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-03-07</depositionDate>
            <releaseDate>2024-06-19</releaseDate>
            <updateDate>2024-06-19</updateDate>
        </keyDates>
        <title>Cryo-ET tilt series of tetracycline-treated FIB-milled E. coli K-12 cells</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0001-5199-5157</authorORCID>
            <firstName>Iskander</firstName>
            <lastName>Khusainov</lastName>
            <organization type="academic">Max Planck Institute of Biophysics</organization>
            <street>Max-von-Laue-str. 3</street>
            <townOrCity>Frankfurt am Main</townOrCity>
            <country>Germany</country>
            <postOrZipCode>60438</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-7397-1321</authorORCID>
            <firstName>Martin</firstName>
            <lastName>Beck</lastName>
            <organization type="academic">Max Planck Institute of Biophysics</organization>
            <street>Max-von-Laue-str. 3</street>
            <townOrCity>Frankfurt am Main</townOrCity>
            <country>Germany</country>
            <postOrZipCode>60438</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0001-5199-5157">Khusainov I</author>
            <author authorORCID="0000-0003-3749-2654">Romanov N</author>
            <author authorORCID="0000-0002-5564-1644">Goemans C</author>
            <author authorORCID="0000-0002-5457-4478">Turoňová B</author>
            <author authorORCID="0000-0003-4388-1349">Zimmerli CE</author>
            <author authorORCID="0000-0002-6049-6664">Welsch S</author>
            <author authorORCID="0000-0002-5190-577X">Langer JD</author>
            <author authorORCID="0000-0002-0797-9018">Typas A</author>
            <author authorORCID="0000-0002-7397-1321">Beck M</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">35.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-11946</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>cell</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-18036</emdbEntry>
            <emdbEntry>EMD-18038</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0001-5199-5157" order="1">Khusainov I</author>
                    <author authorORCID="0000-0003-3749-2654" order="2">Romanov N</author>
                    <author authorORCID="0000-0002-5564-1644" order="3">Goemans C</author>
                    <author authorORCID="0000-0002-5457-4478" order="4">Turoňová B</author>
                    <author authorORCID="0000-0003-4388-1349" order="5">Zimmerli CE</author>
                    <author authorORCID="0000-0002-6049-6664" order="6">Welsch S</author>
                    <author authorORCID="0000-0002-5190-577X" order="7">Langer JD</author>
                    <author authorORCID="0000-0002-0797-9018" order="8">Typas A</author>
                    <author authorORCID="0000-0002-7397-1321" order="9">Beck M</author>
                    <title>Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction</title>
                    <journal>Nature communications</journal>
                    <journalAbbreviation>Nat Commun</journalAbbreviation>
                    <country>Germany</country>
                    <issue>1</issue>
                    <volume>15</volume>
                    <year>2024</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41467-024-49084-5</externalReferences>
                    <externalReferences type="pubmed">38839776</externalReferences>
                </journalCitation>
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        </citationList>
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    <imageSet>
        <name>Cryo-ET tilt series of tetracycline-treated FIB-milled E. coli K-12 cells dataset 1</name>
        <directory>/data/K12_TET-30min_1</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>6</numImagesOrTiltSeries>
        <framesPerImage>10</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>1.697</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>1.697</pixelHeight>
        </dimensions>
        <details>The tilt series stacks of .mrc files were obtained after motion correction of 10 frames, collected using a dose-symmetric scheme starting from a -8 degree pre-tilt and covering a range from -64 to +48 degrees, with a tilt increment of 2 degrees. Each wedge includes 4 tilts.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Cryo-ET tilt series of tetracycline-treated FIB-milled E. coli K-12 cells dataset 2</name>
        <directory>/data/K12_TET-30min_2</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>13</numImagesOrTiltSeries>
        <framesPerImage>10</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>1.697</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>1.697</pixelHeight>
        </dimensions>
        <details>The tilt series stacks of .mrc files were obtained after motion correction of 10 frames, collected using a dose-symmetric scheme starting from a -8 degree pre-tilt and covering a range from -63 to +45 degrees, with a tilt increment of 2 degrees. Each wedge includes 4 tilts.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
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