FIB-SEM datasets of hippocampal neurons from WT and FAM92A1 knockout mice

REL 2023-12-22 2024-12-02 2024-12-02 FIB-SEM datasets of hippocampal neurons from WT and FAM92A1 knockout mice 0000-0003-3862-9237 Helena Vihinen Electron Microscopy Unit, Helsinki Institute of Life Science, Institute of Biotechnology, University of Helsinki Viikinkaari 9 Helsinki Finland 00790 0000-0002-4159-6934 Eija Sofia Jokitalo University of Helsinki Viikinkaari 9 C Helsinki Finland 00790 Vihinen H Jokitalo E Wang L 73.6 10.6019/EMPIAR-12041 FIB-SEM cell Wang L Yang Z Satoshi F Prasanna X Yan Z Vihinen H Chen Y Zhao Y He X Bu Q Li H Zhao Y Jiang L Qin F Dai Y Zhang N Qin M Kuang W Zhao Y Jokitalo E Vattulainen I Kajander T Zhao H Cen X Membrane remodeling by FAM92A1 during brain development regulates neuronal morphology, synaptic function, and cognition Nature communications Nat Commun 1 15 2024 English 10.1038/s41467-024-50565-w 39043703 FIB-SEM dataset of WT mouse hippocampal neurons (DG region) /data/WT_5nm_1950slices micrographs - single frame TIFF TIFF 1950 1 UNSIGNED BYTE 2205 50.0 592 50.0

FIB milling step 5 nm. Images were acquired with pixel size 2.5 nm, xy binned by 2 resulting in voxel size 5x5x5 nm. The SEM (Zeiss Crossbeam 540) was operated at an accelerating voltage of 1.4 kV with 0.3 nA current. The ESB detector was used with a grid voltage of 1000 V. Ion beam milling was performed at an accelerating voltage of 30 kV and current of 700 pA. Images were aligned, grey level adjusted and converted to 8 bit using Zeiss Atlas 5 software.

FIB-SEM dataset of hippocampal neurons (DG region) from FAM92A1 KO mouse /data/HO_5_nm_894slices micrographs - single frame TIFF TIFF 894 1 UNSIGNED BYTE 5143 50.0 816 50.0

FIB milling step 5 nm. Images were acquired with pixel size 2.5 nm, xy binned by 2 resulting in voxel size 5x5x5 nm. The SEM (Zeiss Crossbeam 540) was operated at an accelerating voltage of 1.4 kV with 0.3 nA current. The EsB detector was used with a grid voltage of 1000 V. Ion beam milling was performed at an accelerating voltage of 30 kV and current of 700 pA. Images were aligned, grey level adjusted and converted to 8 bit using Zeiss Atlas 5 software.

raw FIB-SEM dataset of WT mouse hippocampal neurons (DG region) /data/raw_data/WT1_raw_data_Zeiss_crossbeam_540 micrographs - single frame TIFF TIFF 1999 1 UNSIGNED BYTE 8768 25.0 8768 25.0

Raw images from WT-1 sample (10.10.2022) acquired with Zeiss crossbeam 540. Images were acquired with pixel size 2.5 nm, milling thickness 5 nm. The SEM was operated at an accelerating voltage of 1.4 kV with 0.3 nA current. The ESB detector was used with a grid voltage of 1000 V. Ion beam milling was performed at an accelerating voltage of 30 kV and current of 700 pA. Both ESB and Inlens images were collected.

raw FIB-SEM dataset of hippocampal neurons (DG region) from FAM92A1 KO mouse /data/raw_data/HO1_raw_data_Zeiss_crossbeam_540 micrographs - single frame TIFF TIFF 903 1 UNSIGNED BYTE 14976 25.0 14976 25.0

Raw images from HO-1 sample (10.10.2022) acquired with Zeiss crossbeam 540. Images were acquired with pixel size 2.5 nm, milling thickness 5 nm. The SEM was operated at an accelerating voltage of 1.4 kV with 0.3 nA current. The ESB detector was used with a grid voltage of 1000 V. Ion beam milling was performed at an accelerating voltage of 30 kV and current of 700 pA. Both ESB and Inlens images were collected.