Cryo-electron tomography of FIB-milled neuronal synapses in cultured primary neurons

REL 2024-05-30 2025-02-04 2025-02-04 Cryo-electron tomography of FIB-milled neuronal synapses in cultured primary neurons 0000-0001-8231-0341 Richard Held Stanford University SOM 318 Campus Drive Stanford CA United States 94305 0000-0001-5121-2036 Axel Brunger Stanford University 318 Campus Drive Stanford CA United States 94305 Held RG Liang J Brunger AT National Institutes of Health/National Institute of Mental Health (NIH/NIMH) MH063105 United States Howard Hughes Medical Institute (HHMI) N/A United States 47.9 10.6019/EMPIAR-12109 EMDB cell EMD-45161 Held RG Liang J Brunger AT Brunger AT Nanoscale architecture of synaptic vesicles and scaffolding complexes revealed by cryo-electron tomography Proceedings of the National Academy of Sciences of the United States of America Proc Natl Acad Sci U S A United States 27 121 2024 English 10.1073/pnas.2403136121 38923992

Un-aligned tilt-series stacks and associated .rawtlt files. Tilt images in each stack have been motion corrected and are at the physical pixel size (1.7 A/px)

Unaligned tilt-series stacks of cryo-FIB milled neuronal synapses from cultured neurons /data/tilt_series tilt series MRC MRC 14 41 32 BIT FLOAT 5760 1.7 4092 1.7

tilt images are motion corrected (MotionCorr2) sums at physical pixel size of 1.7 A/px.