REL
2024-07-11
2024-08-29
2024-08-29
SPOT-RASTR - a cryo-EM specimen preparation technique that overcomes problems with preferred orientation and the air/water interface
0000-0001-8855-4152
Scott
Stagg
Florida State University
91 Chieftan Way
Tallahassee
FL
United States
32306-4380
0000-0001-5954-8933
Behrouz
Ghazi Esfahani
us
91 Chieftan Way
Tallahassee
FL
United States
32304
Ghazi Esfahani BGE
Randolph PR
Peng RP
Stroupe MES
Grant TG
Stagg SMS
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM148734
United States
304.6
10.6019/EMPIAR-12179
EMDB
molecule
EMD-43514
Esfahani BG
Randolph PS
Peng R
Grant T
Stroupe ME
Stagg SM
SPOT-RASTR - a cryo-EM specimen preparation technique that overcomes problems with preferred orientation and the air/water interface
bioRxiv
PNAS Nexus
United States
2024
English
10.1101/2024.01.24.577038
38501120
The tube picks stack plus the azimuthal average to subtract.
SPOT-RASTR - a cryo-EM specimen preparation technique that overcomes problems with preferred orientation and the air/water interface
/data
picked particles - single frame - unprocessed
MRC
MRCS
230000
80
32 BIT FLOAT
5760
1.44
4092
1.44
The particle stack is the picked lipid nanotube particles. The 3D volume is the tube azimuthal average to be subtracted from the picked particles for further processing based on the described protocol in the SPOT-RASTR paper.
data/J256_stack.star