<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13360" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-10-23</depositionDate>
            <releaseDate>2026-06-17</releaseDate>
            <updateDate>2026-06-17</updateDate>
        </keyDates>
        <title>Single particle cryo-EM structure of the Neisseria meningitidis quinol-dependent nitric oxide reductase (NmqNOR)</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-9014-9380</authorORCID>
            <firstName>Chai</firstName>
            <middleName>C</middleName>
            <lastName>Gopalasingam</lastName>
            <organization type="academic">RIKEN SPring-8 Center</organization>
            <street>1-1-1 Kouto</street>
            <townOrCity>Sayo</townOrCity>
            <stateOrProvince>Hyogo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>679-5148</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-8971-0759</authorORCID>
            <firstName>Tosha</firstName>
            <lastName>Takehiko</lastName>
            <organization type="academic">University of Hyogo</organization>
            <street>3-2-1 Kouto</street>
            <townOrCity>Ako-gun, Kamigori-cho</townOrCity>
            <stateOrProvince>Hyogo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>678-1297</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-9014-9380">Gopalasingam CC</author>
            <author>Egami H</author>
            <author authorORCID="0000-0003-3951-8651">Shigematsu H</author>
            <author>Sakaue M</author>
            <author>Fukumoto K</author>
            <author authorORCID="0000-0002-7265-2804">Gerle C</author>
            <author authorORCID="0000-0002-1311-1768">Yamamoto M</author>
            <author authorORCID="0000-0003-0695-8327">Shiro Y</author>
            <author>Muramoto K</author>
            <author authorORCID="0000-0002-8971-0759">Tosha T</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">3.0</datasetSize>
        <entryDOI>10.6019/EMPIAR-13360</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-60085</emdbEntry>
            <emdbEntry>EMD-60086</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-9014-9380" order="1">Gopalasingam CC</author>
                    <author order="2">Egami H</author>
                    <author authorORCID="0000-0003-3951-8651" order="3">Shigematsu H</author>
                    <author order="4">Sakaue M</author>
                    <author order="5">Fukumoto K</author>
                    <author authorORCID="0000-0002-7265-2804" order="6">Gerle C</author>
                    <author authorORCID="0000-0002-1311-1768" order="7">Yamamoto M</author>
                    <author authorORCID="0000-0003-0695-8327" order="8">Shiro Y</author>
                    <author order="9">Muramoto K</author>
                    <author authorORCID="0000-0002-8971-0759" order="10">Tosha T</author>
                    <title>Structural basis of Neisseria meningitidis quinol dependent nitric oxide reductase activation by dimerization</title>
                    <journal>Communications biology</journal>
                    <journalAbbreviation>Commun Biol</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>9</volume>
                    <year>2026</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s42003-026-09754-0</externalReferences>
                    <externalReferences type="pubmed">41896389</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Single particle cryo-EM structure of the Neisseria meningitidis quinol-dependent nitric oxide reductase (NmqNOR) monomer</name>
        <directory>/data/Monomer</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>8100</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>50</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.752</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.752</pixelHeight>
        </dimensions>
        <details>- Cs: 2.7 mm
- HT: 300 kV
- Dose per frame: 1 e-/A2/frame (total dose 50 e-/A2 over 50 frames)
- A defect file in mrc and txt format (defects_map.mrc or defects_2022-01-20_14.36.29_0120_1_29_0000.txt) is available
- Data collected using 5x5(one exposure per hole) beam-image shift
- gain file: CountCDSRef_2022-01-22_16.49.11_DTM170uMBT3_152-1_0000.mrc</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>1.89 Å single particle cryo-EM structure of the Neisseria meningitidis quinol-dependent nitric oxide reductase (NmqNOR) dimer</name>
        <directory>/data/Dimer</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>7525</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>50</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.752</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.752</pixelHeight>
        </dimensions>
        <details>- Cs: 2.7 mm
- HT: 300 kV
- Dose per frame: 1.02 e-/A2/frame (total dose 51.2 e-/A2 over 50 frames)
- Data collected using 5x5(one exposure per hole) beam-image shift 
- A defect file in mrc and txt format (defects_map.mrc or defects_2022-08-18_15.04.14_WTdimer_500_6_2_193-1_0000.txt) is available
- gain file: CountCDSRef_2022-08-18_15.04.14_WTdimer_500_6_2_193-1_0000.mrc</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
