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        <keyDates>
            <depositionDate>2026-03-04</depositionDate>
            <releaseDate>2026-06-17</releaseDate>
            <updateDate>2026-06-17</updateDate>
        </keyDates>
        <title>Cryo-EM structure of the type I and IVb pilus from enterotoxigenic Escherichia coli</title>
        <correspondingAuthor private="true">
            <firstName>Kazuki</firstName>
            <lastName>Kawahara</lastName>
            <organization type="academic">Research Institute for Microbial Diseases, Osaka University</organization>
            <townOrCity>Suita, Osaka</townOrCity>
            <stateOrProvince>Yamadaoka 3-1</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>565-0871</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <firstName>Hiroya</firstName>
            <lastName>Oki</lastName>
            <organization type="academic">Research Institute for Microbial Diseases, Osaka University</organization>
            <townOrCity>Suita, Osaka</townOrCity>
            <stateOrProvince>Yamadaoka 3-1</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>565-0871</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Kawahara K</author>
            <author>Oki H</author>
            <author>Iimori M</author>
            <author>Muramoto R</author>
            <author>Imai T</author>
            <author>Gerle C</author>
            <author>Shigematsu H</author>
            <author>Matsuda S</author>
            <author>Iida T</author>
            <author>Nakamura S</author>
        </authorsList>
        <grantSupport>
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                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">1.4</datasetSize>
        <entryDOI>10.6019/EMPIAR-13411</entryDOI>
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        <scale>molecule</scale>
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    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-60902</emdbEntry>
            <emdbEntry>EMD-60903</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Kawahara K</author>
                    <author order="2">Oki H</author>
                    <author order="3">Iimori M</author>
                    <author order="4">Muramoto R</author>
                    <author order="5">Imai T</author>
                    <author authorORCID="0000-0002-7265-2804" order="6">Gerle C</author>
                    <author order="7">Shigematsu H</author>
                    <author authorORCID="0000-0001-8430-9723" order="8">Matsuda S</author>
                    <author order="9">Iida T</author>
                    <author order="10">Nakamura S</author>
                    <title>High-resolution cryo-EM analysis visualizes hydrated type I and IV pilus structures from enterotoxigenic Escherichia coli</title>
                    <journal>Structure (London, England : 1993)</journal>
                    <journalAbbreviation>Structure</journalAbbreviation>
                    <country></country>
                    <issue>6</issue>
                    <volume>33</volume>
                    <firstPage>1040</firstPage>
                    <lastPage>1050000.0</lastPage>
                    <year>2025</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1016/j.str.2025.03.010</externalReferences>
                    <externalReferences type="pubmed">40220752</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Cryo-EM structure of the type IVb and I pilus from enterotoxigenic Escherichia coli</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6900</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>50</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.752</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.752</pixelHeight>
        </dimensions>
        <details>- Microscope: CRYO ARM™ 300 electron microscope (JEOL)
- Detector: K3 direct electron detector (Gatan)
- Acceleration voltage (kV): 300
- The pixel size: 0.752 Å/pix.
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (μm): 1.4 - 1.6
- Average electron dose per image (e-/A2): 53.77
- Number of frames per image: 50
- Please use "CountCDSRef_2022-07-08_18.16.20_CFAIII_150-1_0000.mrc" as a gain reference without rotation or flipping in cryoSPARC.</details>
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