REL 2024-12-05 2026-06-17 2026-06-17 Cryo-EM SPA dataset of arginine oxidase from Pseudomonas sp. TRU 7192 0000-0003-1274-1589 Hiroki Yamaguchi Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co. Inc. 1-1 Suzuki-cho, Kawasaki-ku Kawasaki Kanagawa Japan 210-8681 0000-0003-1274-1589 Masayuki Sugiki Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co. Inc. 1-1 Suzuki-cho, Kawasaki-ku Kawasaki-ku Japan 565-0871 Yamaguchi H Takahashi K Numoto N Suzuki H Tatsumi M Kamegawa A Nishikawa K Asano Y Mizukoshi T Miyano H Fujiyoshi Y Sugiki M 916.4 10.6019/EMPIAR-13426 EMDB molecule EMD-36635 Yamaguchi H Takahashi K Numoto N Suzuki H Tatsumi M Kamegawa A Nishikawa K Asano Y Mizukoshi T Miyano H Fujiyoshi Y Sugiki M Open and closed structures of L-arginine oxidase by cryo-electron microscopy and X-ray crystallography Journal of biochemistry J Biochem 2025 English 10.1093/jb/mvae070 39420599 Structure of arginine oxidase from Pseudomonas sp. TRU 7192 /data micrographs - multiframe TIFF TIFF 5035 40 1 40 UNSIGNED 16 BIT INTEGER 3710 0.96 3838 0.96
- Microscope: JEOL JEM-Z320FHC - Acceleration voltage (kV): 300 - Spherical aberration (mm) : 3.4 - Detector: Gatan K2 Summit - Please ignore the pixel size in the header; use 0.96 A/px. - Dose per frame (e-/A^2): 1.74 - Number of frames per image: 40 - Gain reference (MRC format) is included in the folder with the movie files. The gain was created from the dataset by using the 'relion_estimate_gain' command and can be used in RELION without flipping/rotation. In CryoSPARC, select "flip gain ref in Y". - Please ignore the pixel size in the header; use 0.96 A/px.