<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13446" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2026-03-28</depositionDate>
            <releaseDate>2026-08-24</releaseDate>
            <updateDate>2026-08-24</updateDate>
        </keyDates>
        <title>Cryo-EM structure of the IS621 recombinase in complex with bridge RNA, left-half DNA, and right-half DNA</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-2505-1416</authorORCID>
            <firstName>Hiroshi</firstName>
            <lastName>Nishimasu</lastName>
            <organization type="academic">Research Center for Advanced Science and Technology, The University of Tokyo</organization>
            <street>4 Chome-6-1 Komaba</street>
            <townOrCity>Meguro</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>153-8904</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-2505-1416</authorORCID>
            <firstName>Hiroshi</firstName>
            <lastName>Nishimasu</lastName>
            <organization type="academic">Research Center for Advanced Science and Technology, The University of Tokyo</organization>
            <street>4 Chome-6-1 Komaba</street>
            <townOrCity>Meguro</townOrCity>
            <stateOrProvince>Tokyo</stateOrProvince>
            <country>Japan</country>
            <postOrZipCode>153-8904</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-4340-2937">Hiraizumi M</author>
            <author authorORCID="0000-0002-5442-7582">Yamashita K</author>
            <author authorORCID="0000-0002-2505-1416">Nishimasu H</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">3.2</datasetSize>
        <entryDOI>10.6019/EMPIAR-13446</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-65978</emdbEntry>
            <emdbEntry>EMD-65979</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-4340-2937" order="1">Hiraizumi M</author>
                    <author order="2">Athukoralage JS</author>
                    <author authorORCID="0000-0002-1702-4178" order="3">Perry NT</author>
                    <author order="4">Tsujimoto E</author>
                    <author order="5">Shiojiri N</author>
                    <author order="6">Nagahata N</author>
                    <author order="7">Lee L</author>
                    <author order="8">Sun G</author>
                    <author order="9">Durrant MG</author>
                    <author order="10">Chandrasekaran SS</author>
                    <author authorORCID="0000-0001-7915-1685" order="11">Konermann S</author>
                    <author authorORCID="0000-0002-5442-7582" order="12">Yamashita K</author>
                    <author authorORCID="0000-0002-9380-2648" order="13">Hsu PD</author>
                    <author authorORCID="0000-0002-2505-1416" order="14">Nishimasu H</author>
                    <title>Structural mechanism governing the directionality of bridge recombination</title>
                    <journal>Nature</journal>
                    <journalAbbreviation>Nature</journalAbbreviation>
                    <country></country>
                    <year>2026</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1038/s41586-026-10903-y</externalReferences>
                    <externalReferences type="pubmed">42587149</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>EMD-65978 (pre)</name>
        <directory>/data/pre</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6558</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>Gain: data_1/GridSquare_26880948/Data/FoilHole_26915916_Data_26915121_26915123_20231226_165524_gain.tiff</details>
        <segmentationList/>
        <micrographsFilePattern>data/pre/data_*/*/Data/*fractions.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>EMD-65979 (post)</name>
        <directory>/data/post</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>7668</numImagesOrTiltSeries>
        <framesPerImage>50</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>5760</imageWidth>
            <pixelWidth>0.83</pixelWidth>
            <imageHeight>4092</imageHeight>
            <pixelHeight>0.83</pixelHeight>
        </dimensions>
        <details>data_1 and data_2 were collected from different grids.
gain: data_1/GridSquare_24969694/Data/FoilHole_25057155_Data_25055102_25055104_20240125_225422_gain.tiff</details>
        <segmentationList/>
        <micrographsFilePattern>data/post/data_*/*/Data/*fractions.tiff</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
