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2026-03-21
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2026-07-02
Cryo-EM tomography raw tilt series data of human apo-ferrtin data collection using continuous recording based beam image shift (CR-BIS)
0000-0002-0114-0270
Xinzheng
Zhang
Institute of Biophysics, Chinese Academy of Sciences
15 Datun Road
Beijing
Beijing
China
100101
0000-0002-0114-0270
Xinzheng
Zhang
State Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Beijing
China
100101
Yang Q
Huang XJ
Wu CL
Zeng Y
Zhang XZ
National Natural Science Foundation of China (NSFC)
32325027
China
National Natural Science Foundation of China (NSFC)
31930069
China
120.3
10.6019/EMPIAR-13494
EMDB
molecule
EMD-68817
Yang Q
Huang XJ
Wu CL
Zeng Y
Zhang XZ
Ultra-Rapid Cryo-EM Data Acquisition Method Enabled by Continuous Recording Based Beam Image Shift
English
The raw tilt series data are eer format files without any realignment collected using continuous recording based beam image shift (CR-BIS) method.
Ferritin cryo-ET dataset acquired with CR-BIS method on Falcon4 (unsplit whole EER files)
/data
micrographs - multiframe
EER
EER
770
70
UNSIGNED BYTE
4096
0.731
4096
0.731
This dataset contains ferritin cryo-ET data collected using the Falcon4 camera. The uploaded directory includes the original, unprocessed whole EER files as generated by the CR-BIS acquisition scheme. For the images of first 5 tilt angle contain 70 eer frames. For images of the rest of tilt angles, each whole EER file can be split into 4 individual single-particle images corresponding to different target positions using the provided Python scripts. Please note that running the splitting script automatically deletes the original whole EER files.
data/CR-BIS-Tomo_Falcon4/Falcon4_*.eer