REL 2026-01-06 2026-04-28 2026-04-28 0000-0001-7497-0972 Rhiju Das Department of Biochemistry, Stanford University; Howard Hughes Medical Institute 279 Campus Dr W Stanford CA United States 94305 0000-0002-8910-3078 Wah Chiu Department of Bioengineering, James H. Clark Center, Stanford University; CryoEM and Bioimaging Division, Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory 318 CAMPUS DR Stanford CA United States 94305 Zhang K Zheludev IN Hagey RJ Haslecker R Hou YJ Kretsch R Pintilie GD Rangan R Kladwang W Li S Wu MT Pham EA Bernardin-Souibgui C Baric RS Sheahan TP D'Souza V Glenn JS Chiu W Das R National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM122579 United States Howard Hughes Medical Institute (HHMI) N/A United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) U24GM129541 United States 13.7 10.6019/EMPIAR-13496 EMDB molecule EMD-22296 EMD-22297 Zhang K Zheludev IN Hagey RJ Haslecker R Hou YJ Kretsch R Pintilie GD Rangan R Kladwang W Li S Wu MT Pham EA Bernardin-Souibgui C Baric RS Sheahan TP D'Souza V Glenn JS Chiu W Das R Nature structural & molecular biology Nat Struct Mol Biol 9 28 747 754 2021 English 10.1038/s41594-021-00653-y 34426697 Unaligned multiframe micrographs of SARS-CoV-2 Frameshift Stimulation Element /data/FSE_1 micrographs - multiframe MRC MRC 10010 30 UNSIGNED BYTE variable 0.82 variable 0.82

The final datasets in this study were imaged in a Titan Krios cryo-electron microscope (Thermo Fisher Scientific) operated at 300 kV with a GIF energy filter (Gatan) at a magnification of ×165,000 (corresponding to a calibrated sampling of 0.82 Å per pixel) for both samples. Micrographs were recorded by EPU software (Thermo Fisher Scientific) with a Gatan K2 Summit direct electron detector, where each image was composed of 30 individual frames with an exposure time of 6 s and an exposure rate of 8.3 electrons per second per Å2.

data/FSE_1/Images-Disc1/G*/Data/F*Data*-*.mrc Unaligned multiframe micrographs of SARS-CoV-2 Frameshift Stimulation Element /data/FSE_2 micrographs - multiframe MRC MRC 13155 30 UNSIGNED BYTE variable 0.82 variable 0.82

The final datasets in this study were imaged in a Titan Krios cryo-electron microscope (Thermo Fisher Scientific) operated at 300 kV with a GIF energy filter (Gatan) at a magnification of ×165,000 (corresponding to a calibrated sampling of 0.82 Å per pixel) for both samples. Micrographs were recorded by EPU software (Thermo Fisher Scientific) with a Gatan K2 Summit direct electron detector, where each image was composed of 30 individual frames with an exposure time of 6 s and an exposure rate of 8.3 electrons per second per Å2.

data/FSE_2/Images-Disc1/G*/Data/*Data*-*mrc Unaligned multiframe micrographs of SARS-CoV-2 Frameshift Stimulation Element Tagged with ATP-TTR3 nanostructure /data/FSE_ATP_TTR3_1 micrographs - multiframe MRC MRC 2714 30 UNSIGNED BYTE variable 0.82 variable 0.82

The final datasets in this study were imaged in a Titan Krios cryo-electron microscope (Thermo Fisher Scientific) operated at 300 kV with a GIF energy filter (Gatan) at a magnification of ×165,000 (corresponding to a calibrated sampling of 0.82 Å per pixel) for both samples. Micrographs were recorded by EPU software (Thermo Fisher Scientific) with a Gatan K2 Summit direct electron detector, where each image was composed of 30 individual frames with an exposure time of 6 s and an exposure rate of 8.3 electrons per second per Å2.

data/FSE_ATP_TTR3_1/Images-Disc1/G*/Data/*Data*-*mrc Unaligned multiframe micrographs of SARS-CoV-2 Frameshift Stimulation Element Tagged with ATP-TTR3 nanostructure /data/FSE_ATP_TTR3_2 micrographs - multiframe MRC MRC 9455 30 UNSIGNED BYTE variable 0.82 variable 0.82

The final datasets in this study were imaged in a Titan Krios cryo-electron microscope (Thermo Fisher Scientific) operated at 300 kV with a GIF energy filter (Gatan) at a magnification of ×165,000 (corresponding to a calibrated sampling of 0.82 Å per pixel) for both samples. Micrographs were recorded by EPU software (Thermo Fisher Scientific) with a Gatan K2 Summit direct electron detector, where each image was composed of 30 individual frames with an exposure time of 6 s and an exposure rate of 8.3 electrons per second per Å2.

data/FSE_ATP_TTR3_2/Images-Disc1/G*/Data/*Data*-*mrc