REL 2025-10-17 2026-10-06 2026-10-06 Cryo-EM structure of the hTUT4_mini:Lin28A:pre-let-7g_UUU complex 0000-0002-7374-3898 Kozo Tomita Department of Computational Biology and Medical Sciences, The University of Tokyo 5-1-5 Kashiwanoha Kashiwa-shi, Chiba-ken Japan 277-8562 0000-0002-7374-3898 Kozo Tomita Department of Computational Biology and Medical Sciences, The University of Tokyo 5-1-5 Kashiwanoha Kashiwa-shi, Chiba-ken Japan 277-8562 Han X Yamashita S Tomita K 7.7 10.6019/EMPIAR-13721 EMDB molecule EMD-65642 EMD-65643 Han X Yamashita S Tomita K Mechanistic insights into Lin28-dependent oligo-uridylylation of pre-let-7 by TUT4 Nucleic acids research Nucleic Acids Res 1 54 2026 English 10.1093/nar/gkaf1421 41521656 20240719_Tomita_TUT4_3_data /data micrographs - multiframe TIFF TIFF 4296 50 1 50 UNSIGNED BYTE 5760 variable 4092 variable
- Microscope: FEI TITAN Krios - Acceleration voltage (kV): 300 - Spherical aberration (Cs) : 2.7 - Defocus (nm): -800 to -1800 - Magnification:105000 - Detector: Gatan K3 BIOQUANTUM - Average electron dose per image (e-/A2): 50 - Number of frames per image: 50 - The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value. - Please use 'gain.mrc' as a gain reference with y-flipping for CryoSPARC.
 20240724_Tomita_TUT4_2_data /data micrographs - multiframe TIFF TIFF 9977 50 1 50 UNSIGNED BYTE 5760 variable 4092 variable
- Microscope: FEI TITAN Krios - Acceleration voltage (kV): 300 - Spherical aberration (Cs) : 2.7 - Defocus (nm): -800 to -1800 - Magnification:105000 - Detector: Gatan K3 BIOQUANTUM - Average electron dose per image (e-/A2): 50 - Number of frames per image: 50 - The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value. - Please use 'gain.mrc' as a gain reference with y-flipping for CryoSPARC.
 20240808_Tomita_TUT4_1_data /data micrographs - multiframe TIFF TIFF 9818 50 1 50 UNSIGNED BYTE 5760 variable 4092 variable
- Microscope: FEI TITAN Krios - Acceleration voltage (kV): 300 - Spherical aberration (Cs) : 2.7 - Defocus (nm): -800 to -1800 - Magnification:105000 - Detector: Gatan K3 BIOQUANTUM - Average electron dose per image (e-/A2): 50 - Number of frames per image: 50 - The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value. - Please use 'gain.mrc' as a gain reference with y-flipping for CryoSPARC.
 20241129_Tomita_TUT4_gUUU_Au_1_data /data micrographs - multiframe TIFF TIFF 6382 50 1 50 UNSIGNED BYTE 5760 variable 4092 variable
- Microscope: FEI TITAN Krios - Acceleration voltage (kV): 300 - Spherical aberration (Cs) : 2.7 - Defocus (nm): -800 to -1800 - Magnification:105000 - Detector: Gatan K3 BIOQUANTUM - Average electron dose per image (e-/A2): 50 - Number of frames per image: 50 - The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value. - Please use 'gain.mrc' as a gain reference with y-flipping for CryoSPARC.
 20241129_Tomita_TUT4_gUUU_Au_2_data /data micrographs - multiframe TIFF TIFF 7290 50 1 50 UNSIGNED BYTE 5760 variable 4092 variable
- Microscope: FEI TITAN Krios - Acceleration voltage (kV): 300 - Spherical aberration (Cs) : 2.7 - Defocus (nm): -800 to -1800 - Magnification:105000 - Detector: Gatan K3 BIOQUANTUM - Average electron dose per image (e-/A2): 50 - Number of frames per image: 50 - The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value. - Please use 'gain.mrc' as a gain reference with y-flipping for CryoSPARC.