REL
2026-07-27
2026-08-13
2026-08-13
Cryo-EM structure of TrhO from B. subtilis complexed with tRNA Ala
0000-0001-6785-616X
Gyuhyeok
Cho
Department of Chemistry, Gwangju Institute of Science and Technology
123, Cheomdangwagi-ro, Buk-gu
Jeonnam-Gwangju
Korea, South
61005
0000-0001-5213-9299
Jungwook
Kim
Department of Chemistry, Gwangju Institute of Science and Technology
123, Cheomdangwagi-ro, Buk-gu
Jeonnam-Gwangju
Korea, South
61005
Shin K
Kim J
National Research Foundation (NRF, Korea)
RS-2024-00411137
Korea, South
National Research Foundation (NRF, Korea)
RS-2024-00440614
Korea, South
National Research Foundation (NRF, Korea)
RS-2025-23323757
Korea, South
10.8
10.6019/EMPIAR-13850
EMDB
molecule
EMD-61148
Shin K
Han DB
Kim HW
Kim J
Unconventional monooxygenation by the O<sub>2</sub>-dependent tRNA wobble uridine hydroxylase TrhO
Nature chemical biology
Nat Chem Biol
6
22
886
894
2026
English
10.1038/s41589-025-02129-2
41554887
Videos were collected using EPU (version 2.14.0; Thermo Fisher Scientific) in counting mode with a total dose of 60 e− per Å2 for a 7.15-s exposure. A total of 10,314 videos were recorded in EER format at a nominal magnification of ×130,000, corresponding to a physical pixel size of 0.9013 Å, with a defocus range from −0.6 to −2.1 μm.
Cryo-EM structure of TrhO from B. subtilis complexed with tRNA Ala
/data
micrographs - multiframe
EER
EER
10314
60
BIT
4096
0.9013
4096
0.9013
Videos were collected using EPU (version 2.14.0; Thermo Fisher Scientific) in counting mode with a total dose of 60 e− per Å2 for a 7.15-s exposure. A total of 10,314 videos were recorded in EER format at a nominal magnification of ×130,000, corresponding to a physical pixel size of 0.9013 Å, with a defocus range from −0.6 to −2.1 μm.
data/GridSquare_*/Data/*.eer