REL 2021-03-29 2022-01-24 2022-01-24 0000-0003-3153-9490 Axel Innis ARNA,INSERM, IECB 2, rue Robert Escarpit Pessac Gironde France 33600 0000-0003-3153-9490 Axel Innis ARNA,INSERM, IECB 2, rue Robert Escarpit Pessac Gironde France 33600 van der Stel AX Gordon ER Sengupta A Martínez AK Klepacki D Perry TN Herrero Del Valle A Vázquez-Laslop N Sachs MS Cruz-Vera LR Innis CA 6.9 10.6019/EMPIAR-10695 EMDB EMD-12693 EMD-12694 EMD-12695 van der Stel AX Gordon ER Sengupta A Martínez AK Klepacki D Perry TN Herrero Del Valle A Vázquez-Laslop N Sachs MS Cruz-Vera LR Innis CA Nature communications Nat Commun 1 12 2021 English 10.1038/s41467-021-25663-8 34504068 Raw multiframe micrographs of TnaC-WT-70S ribosome stalled on L-Trp /data/TnaC_WT_raw_micrographs micrographs - multiframe TIFF TIFF 8964 35 1 35 UNSIGNED 16 BIT INTEGER 3710 0.907 3838 0.907

Raw micrographs collected on a Talos Arctica (IECB-Bordeaux) with a nominal pixelsize of 0.93A/px (calibrated 0.907A/px) of the TnaC-WT-70S sample as described in the original reference. These micrographs were used to reconstruct the map EMDB-12693 and corresponding molecular model PDB-7O19. This map and model contain the 70S ribosome stalled on TnaC in response to L-tryptophan All image processing has been performed using RELION3.1

data/TnaC_WT_raw_micrographs/Ribo_*.tif Raw multiframe micrographs of TnaC-R23F-70S ribosome stalled on L-Trp (dataset B) /data/TnaC_R23F_Talos_IECB micrographs - multiframe TIFF TIFF 8743 35 1 35 UNSIGNED 16 BIT INTEGER 3710 0.907 3838 0.907

Raw micrographs collected on a Talos Arctica (IECB-Bordeaux) with a nominal pixsize of 0.93A/px (calibrated 0.907A/px) of the TnaC-(R23F)-70S sample as described in the original reference. This dataset (TnaC-(R23F)-70S_B) was used together with the dataset (TnaC-(R23F)-70S_A) to reconstruct the map EMDB-12695 and corresponding molecular model PDB-7O1C. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan in complex with release factor 2 (RF2) in the A-site in a near-active conformation. All image processing has been performed using RELION3.1

data/TnaC_R23F_Talos_IECB/R23F_*.tif Raw multiframe micrographs of TnaC-R23F-70S ribosome stalled on L-Trp (dataset A) /data/TnaC_R23F_KRIOS_ESRF_raw_micrographs micrographs - multiframe MRC MRC 7867 40 1 40 UNSIGNED BYTE 3838 0.835 3710 0.835

Raw micrographs collected on a Titan KRIOS (ESRF-Grenoble) with a nominal pixsize of 0.827A/px (calibrated 0.835A/px) of the TnaC-(R23F)-70S sample as described in the original reference. This dataset (TnaC-(R23F)-70S_B) was used to reconstruct the map EMDB-12694 and corresponding molecular model PDB-7O1A. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan. In addition, this dataset was also used together with the dataset (TnaC-(R23F)-70S_B) to reconstruct the map EMDB-12695 and corresponding molecular model PDB-7O1C. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan in complex with release factor 2 (RF2) in the A-site in a near-active conformation. All image processing has been performed using RELION3.1

data/TnaC_R23F_KRIOS_ESRF_raw_micrographs/FoilHole_*.mrc