REL
2023-08-02
2024-06-27
2024-06-27
Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I
0000-0001-8667-6797
Judy
Hirst
MRC Mitochondrial Biology Unit
Hills Road
Cambridge
Cambridgeshire
United Kingdom
CB2 0XY
0000-0001-8667-6797
Judy
Hirst
MRC Mitochondrial Biology Unit
Hills Road
Cambridge
Cambridgeshire
United Kingdom
CB2 0XY
Grba DN
Hirst J
Medical Research Council (MRC, United Kingdom)
MC_U105663141
United Kingdom
Medical Research Council (MRC, United Kingdom)
MC_UU_00015/2
United Kingdom
3.3
10.6019/EMPIAR-11662
EMDB
molecule
EMD-10815
EMD-10816
Grba DN
Hirst J
Mitochondrial complex I structure reveals ordered water molecules for catalysis and proton translocation
Nature structural & molecular biology
Nat Struct Mol Biol
10
27
892
900
2020
English
10.1038/s41594-020-0473-x
32747785
Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM. Frozen gold grids treated with PEGylated thiol reagent. Images (MRC) were collected with a 300 keV FEI Titan Krios on a Gatan K2 Summit detector, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 10 s exposures were captured in 25 frames with a total dose of ~44 e/Å-2. A pixel size of 1.055 Å/pixel was used.
Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM.
/data
micrographs - multiframe
MRC
MRC
2540
25
1
25
32 BIT FLOAT
3838
1.055
3710
1.055
Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM. Frozen gold grids treated with PEGylated thiol reagent. Images (MRC) were collected with a 300 keV FEI Titan Krios on a Gatan K2 Summit detector, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 10 s exposures were captured in 25 frames with a total dose of ~44 e/Å-2. A pixel size of 1.055 Å/pixel was used.