REL
2024-02-18
2024-09-03
2024-09-03
Volume correlative light X-ray and electron microscopy (CLXEM) of mouse cremaster muscle endothelial cells
0000-0001-6072-6864
Azumi
Yoshimura
Yamaguchi University
Ube
Japan
7558505
0000-0001-6072-6864
Azumi
Yoshimura
Yamaguchi University
Ube
Japan
7558505
Reglero-Real N
Pérez-Gutiérrez L
Saleeb R
Nourshargh S
Collinson L
Yoshimura A
Wellcome Trust
098291/Z/12/Z
United Kingdom
Ministerio de Ciencia e Innovacion (MCIN)
RYC2021-031221-I
Spain
Cancer Research UK
CC1076
United Kingdom
Medical Research Council (MRC, United Kingdom)
CC1076
United Kingdom
Wellcome Trust
CC1076
United Kingdom
79.2
10.6019/EMPIAR-11958
CLEM
tissue
Reglero-Real N
Pérez-Gutiérrez L
Saleeb RS
Nourshargh S
Collinson L
Yoshimura A
Volume correlative light X-ray and electron microscopy (CLXEM) of endothelial cells in mouse tissue
STAR Protocols
10.1016/j.xpro.2024.103257
S-BSST1339
X-ray micro-computed tomography (µCT) of resin-embedded mouse cremaster muscle tissue
/data/micro-CT_reconstructed
reconstructed volumes
TIFF
TIFF
1
1012
UNSIGNED BYTE
1004
74181.0
1024
74181.0
Mouse cremaster muscle tissue containing the region of interest previously imaged by confocal laser scanning microscopy. Light microscopy data is available in BioStudies.
X-ray µCT of resin-embedded mouse cremaster muscle tissue after first trimming around the region of interest
/data/micro-CT_reconstructed_Trim1
reconstructed volumes
TIFF
TIFF
1
1003
UNSIGNED BYTE
992
32635.0
1015
32635.0
Mouse cremaster muscle tissue containing the region of interest previously imaged by confocal laser scanning microscopy. Light microscopy data is available in BioStudies.
X-ray µCT of resin-embedded mouse cremaster muscle tissue after final trimming before SBF-SEM
/data/micro-CT_reconstructed_Trim2
reconstructed volumes
TIFF
TIFF
1
997
UNSIGNED BYTE
992
10313.0
1015
10313.0
Mouse cremaster muscle tissue containing the region of interest previously imaged by confocal laser scanning microscopy. Light microscopy data is available in BioStudies.
SBF-SEM of the target endothelial cells identified by correlation of X-ray and light microscopy
/data/SBFSEM_processed
micrographs - multiframe
TIFF
TIFF
1
629
UNSIGNED BYTE
9348
84.4
13946
500.0
Data set of two SBF SEM acquisitions combined, aligned and processed as a tiff stack at the original resolution of acquisition.