<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12070" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-04-17</depositionDate>
            <releaseDate>2024-06-16</releaseDate>
            <updateDate>2024-06-16</updateDate>
        </keyDates>
        <title>Hypopseudouridylated yeast 80S bound with Taura syndrome virus (TSV) internal ribosome entry site (IRES) [ three datasets, in TIF format]</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-2046-9861</authorORCID>
            <firstName>Hong</firstName>
            <lastName>Li</lastName>
            <organization type="academic">Institute of Molecular Biophysics, Florida State University</organization>
            <street>91 Chieftan Way</street>
            <townOrCity>Tallahassee</townOrCity>
            <stateOrProvince>Florida</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>32306</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-2046-9861</authorORCID>
            <firstName>Hong</firstName>
            <lastName>Li</lastName>
            <organization type="academic">Institute of Molecular Biophysics, Florida State University</organization>
            <street>91 Chieftan Way</street>
            <townOrCity>Tallahassee</townOrCity>
            <stateOrProvince>Florida</stateOrProvince>
            <country>United States</country>
            <postOrZipCode>32306</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-0485-2780">Zhao Y</author>
            <author authorORCID="0000-0002-4473-3850">Rai J</author>
            <author authorORCID="0000-0003-2046-9861">Li H</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>National Institutes of Health (NIH)</fundingBody>
                <code>R01GM124622</code>
                <country>United States</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="TB">16.0</datasetSize>
        <entryDOI>10.6019/EMPIAR-12070</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-28632</emdbEntry>
            <emdbEntry>EMD-28643</emdbEntry>
            <emdbEntry>EMD-28636</emdbEntry>
            <emdbEntry>EMD-28610</emdbEntry>
            <emdbEntry>EMD-28635</emdbEntry>
            <emdbEntry>EMD-28642</emdbEntry>
            <emdbEntry>EMD-28633</emdbEntry>
            <emdbEntry>EMD-28634</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0003-0485-2780" order="1">Zhao Y</author>
                    <author authorORCID="0000-0002-4473-3850" order="2">Rai J</author>
                    <author authorORCID="0000-0003-2046-9861" order="3">Li H</author>
                    <title>Regulation of translation by ribosomal RNA pseudouridylation</title>
                    <journal>Science advances</journal>
                    <journalAbbreviation>Sci Adv</journalAbbreviation>
                    <country></country>
                    <issue>33</issue>
                    <volume>9</volume>
                    <year>2023</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1126/sciadv.adg8190</externalReferences>
                    <externalReferences type="pubmed">37595043</externalReferences>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unalinged multi-frame micrographs of Hypopseudouridylated yeast 80S bound with TSV-IRES RNA [initiation complex]</name>
        <directory>/data/Initiation_Complex</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>3878</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.53</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.53</pixelHeight>
        </dimensions>
        <details>Gain ref is save in data/Initiation_Complex/gain/ folder. 
Total dose 60 e.
no rotation, Flip upsidedown for motioncor in RELION; no rotation, no flip in CryoSPARC.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unalinged multi-frame micrographs of Hypopseudouridylated yeast 80S bound with TSV-IRES RNA [sordarin bound translocation complex]</name>
        <directory>/data/Translocation_Complex_with_Sordarin</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6082</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.53</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.53</pixelHeight>
        </dimensions>
        <details>Gain ref is save in data/Initiation_Complex/gain/ folder. 
Total dose 60 e.
no rotation, Flip upsidedown for motioncor in RELION; no rotation, no flip in CryoSPARC.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>Unalinged multi-frame micrographs of Hypopseudouridylated yeast 80S bound with TSV-IRES RNA [termination complex]</name>
        <directory>/data/Termination_Complex</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>16443</numImagesOrTiltSeries>
        <framesPerImage>60</framesPerImage>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>11520</imageWidth>
            <pixelWidth>0.53</pixelWidth>
            <imageHeight>8184</imageHeight>
            <pixelHeight>0.53</pixelHeight>
        </dimensions>
        <details>Gain ref is save in data/Initiation_Complex/gain/ folder. 
Total dose 60 e.
no rotation, Flip upsidedown for motioncor in RELION; no rotation, no flip in CryoSPARC.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
