<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-12190" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-06-20</depositionDate>
            <releaseDate>2024-09-23</releaseDate>
            <updateDate>2024-09-23</updateDate>
        </keyDates>
        <title>FAST-EM array tomography data of wild-type MCF-7 cells prepared with the rOTO protocol and stained with uranyl acetate</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0003-4457-9627</authorORCID>
            <firstName>Arent</firstName>
            <lastName>Kievits</lastName>
            <organization type="academic">Delft University of Technology, The Netherlands</organization>
            <street>Lorentzweg 1</street>
            <townOrCity>Delft</townOrCity>
            <stateOrProvince>Zuid-Holland</stateOrProvince>
            <country>Netherlands</country>
            <postOrZipCode>2628 CJ</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0003-4457-9627</authorORCID>
            <firstName>Arent</firstName>
            <lastName>Kievits</lastName>
            <organization type="academic">Delft University of Technology, The Netherlands</organization>
            <street>Lorentweg 1</street>
            <townOrCity>Delft</townOrCity>
            <stateOrProvince>Zuid-Holland</stateOrProvince>
            <country>Netherlands</country>
            <postOrZipCode>2628 CJ</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0003-4457-9627">Kievits AJ</author>
            <author authorORCID="0000-0003-0699-0001">Duinkerken BHP</author>
            <author authorORCID="0000-0002-5887-2069">Lane R</author>
            <author authorORCID="0000-0001-8618-8451">de Heus C</author>
            <author>van Beijeren Bergen en Henegouwen D</author>
            <author>Höppener T</author>
            <author>Wolters AHG</author>
            <author authorORCID="0000-0003-2654-9117">Liv N</author>
            <author authorORCID="0000-0001-5105-5915">Giepmans BNG</author>
            <author authorORCID="0000-0003-4539-8772">Hoogenboom JP</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody>Netherlands Organisation for Scientific Research (NWO)</fundingBody>
                <code>184.034.014</code>
                <country>Netherlands</country>
            </grantReference>
            <grantReference>
                <fundingBody>Netherlands Organisation for Scientific Research (NWO)</fundingBody>
                <code>ENPPS.LIFT.019.030</code>
                <country>Netherlands</country>
            </grantReference>
            <grantReference>
                <fundingBody>Netherlands Organisation for Scientific Research (NWO)</fundingBody>
                <code>OCENW.KLEIN.414</code>
                <country>Netherlands</country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">561.3</datasetSize>
        <entryDOI>10.6019/EMPIAR-12190</entryDOI>
        <experimentType>ATUM-SEM</experimentType>
        <scale>cell</scale>
    </admin>
    <crossReferences>
        <citationList>
            <universalCitation>
                <journalCitation published="false" preprint="false">
                    <author authorORCID="0000-0003-4457-9627" order="1">Kievits AJ</author>
                    <author authorORCID="0000-0003-0699-0001" order="2">Duinkerken BHP</author>
                    <author authorORCID="0000-0002-5887-2069" order="3">Lane R</author>
                    <author authorORCID="0000-0001-8618-8451" order="4">de Heus C</author>
                    <author order="5">van Beijeren Bergen en Henegouwen D</author>
                    <author order="6">Höppener T</author>
                    <author order="7">Wolters AHG</author>
                    <author authorORCID="0000-0003-2654-9117" order="8">Liv N</author>
                    <author authorORCID="0000-0001-5105-5915" order="9">Giepmans BNG</author>
                    <author authorORCID="0000-0003-4539-8772" order="10">Hoogenboom JP</author>
                    <editor authorORCID="0000-0003-4457-9627" order="1">Kievits AJ</editor>
                    <editor authorORCID="0000-0003-4539-8772" order="2">Hoogenboom JP</editor>
                    <title>FAST-EM array tomography: a workflow for multibeam volume electron microscopy</title>
                    <journal>Methods in Microscopy</journal>
                    <journalAbbreviation>MiM</journalAbbreviation>
                    <country>Germany</country>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Raw FAST-EM array tomography electron micrographs from wild-type MCF-7 cells stained with rOTO protocol and uranyl acetate</name>
        <directory>/data/20231107_MCF7_UAC</directory>
        <category>micrographs - single frame</category>
        <headerFormat>XML</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>5595</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>6400</imageWidth>
            <pixelWidth>40.0</pixelWidth>
            <imageHeight>6400</imageHeight>
            <pixelHeight>40.0</pixelHeight>
        </dimensions>
        <details>Raw serial-section (array tomography) data of MCF-7 cells acquired with FAST-EM, ordered per section. Each section directory (SXX) contains 10x10 images covering an area of approximately 240 x 240 microns, with 6.25% overlap between images. Images were acquired with 20 us dwell time.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
