REL 2024-08-30 2025-03-19 2025-03-19 Cryo-electron tomography of optogenetically-induced lamellipodia 0000-0002-0629-0518 Nakata Takao Tokyo Medical and Dental University 1-5-45, Yushima Bunkyo-ku Tokyo Japan 113-8510 0000-0002-0629-0518 Nakata Takao Tokyo Medical and Dental University 1-5-45, Yushima Bunkyo-ku Tokyo Japan 113-8510 Inaba H Imasaki T Aoyama K Yoshihara S Takasaki H Kato T Goto H Mitsuoka K Nitta R Nakata T 57.8 10.6019/EMPIAR-12292 EMDB cell EMD-61088 EMD-61091 EMD-61092 EMD-61093 Inaba H Imasaki T Aoyama K Yoshihara S Takazaki H Kato T Goto H Mitsuoka K Nitta R Nakata T Cryo-electron tomography of the actin cytoskeleton and membrane organization during lamellipodia formation using optogenetics bioRxiv 2024 10.1101/2024.08.13.607852 Final models /data/Final_model reconstructed volumes MRC MRC 16 1 32 BIT FLOAT variable variable variable variable
- Titan Krios - 300 kV - 5 μm Cs corrected (CEOS, GmbH) - BioQuantam K3 direct ditector, energy filter (slit width of 20 eV) - 1.44 e/A2/frame - no gain reference file - EMD-61088：Cell1_Position1 - EMD-61091：Cell1_Position4 - EMD-61092：Cell2_Position1 - EMD-61093：Cell2_Position2
 Tilt series /data/Tilt_series tilt series MRC MRC 16 69 UNSIGNED 16 BIT INTEGER 5760 variable 4092 variable