Cryo-EM structures of TUG-1375/4-CMTB-bound active FFA2 and GLPG0974-bound inactive FFA2

REL 2024-12-05 2025-06-27 2025-06-27 Cryo-EM structures of TUG-1375/4-CMTB-bound active FFA2 and GLPG0974-bound inactive FFA2 0000-0002-1941-5535 Hideaki E Kato Research Center for Advanced Science and Technology, The University of Tokyo 4-6-1 Komaba Meguro Tokyo Japan 153-8904 0000-0002-1941-5535 Hideaki E Kato Research Center for Advanced Science and Technology, The University of Tokyo 4-6-1 Komaba Meguro Tokyo Japan 153-8904 Kugawa M Kawakami K Kise R Suomivuori CM Tsujimura M Kobayashi K Kojima A Inoue WJ Fukuda M Matsui TE Fukunaga A Kobayashi J Kim S Ikeda H Yamashita K Saito K Ishikita H Dror RO Inoue A Kato HE 5.7 10.6019/EMPIAR-12493 EMDB molecule EMD-39003 EMD-39004 Kise R Kojima A Inoue WJ Matsui TE Fukunaga A Kobayashi J Kim S Ikeda H Yamashita K Saito K Ishikita H Dror RO Inoue A Kato HE Kawakami K Suomivuori CM Tsujimura M Kobayashi K Kojima A Fukuda M Matsui TE Fukunaga A Koyanagi J Kim S Ikeda H Saito K Inoue A Structural insights into lipid chain-length selectivity and allosteric regulation of FFA2 Nature communications Nat Commun 1 16 2025 English 10.1038/s41467-025-57983-4 40140663 TUG-1375 and 4-CMTB-bound human FFA2 in complex with Gi /data/FFA2_active micrographs - multiframe TIFF TIFF 8758 48 UNSIGNED BYTE 5760 0.83 4092 0.83

- Please use "gain_reference/movie_gain.mrc" as gain reference file. - Please ignore the pixel size in the header (1.00 Å/px); use 0.83 Å/px. - Raw pixel size: 0.83 A - Accelerating voltage: 300 kV - Spherical aberration: 2.7 mm - Total exposure dose: 45.579 e/A^2

GLPG0974-bound human FFA2 /data/FFA2_inactive/dataset1 micrographs - multiframe TIFF TIFF 7474 48 UNSIGNED BYTE 5760 0.83 4092 0.83

GLPG0974-bound human FFA2 /data/FFA2_inactive/dataset2 micrographs - multiframe TIFF TIFF 10021 48 UNSIGNED BYTE 5760 0.83 4092 0.83