REL
2025-02-24
2025-10-30
2025-10-30
Pichia pastoris mitochondrial complex I reconstituted into proteoliposomes
0000-0003-2915-951X
Daniel
Grba
University of Cambridge
Hills Road
Cambridge
United Kingdom
CB2 0XY
0000-0001-8667-6797
Judy
Hirst
University of Cambridge
Hills Road
Cambridge
United Kingdom
CB2 0XY
Grba D.N.
Hirst J.
Medical Research Council (MRC, United Kingdom)
MC_UU_00015/2
United Kingdom
880.7
10.6019/EMPIAR-12582
EMDB
molecule
EMD-52893
EMD-52892
EMD-52891
EMD-52890
Lee CS
Grba DN
Wright JJ
Ivanov BS
Hirst J
Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit
Science advances
Sci Adv
40
11
2025
English
10.1126/sciadv.adz0693
41032597
Unaligned multi-frame micrographs of Pichia pastoris (Komagataella phaffii) mitochondrial complex I from an as-prepared sample solubilised in LMNG and reconstituted in to proteoliposomes.
/data
micrographs - multiframe
MRC
MRC
854
33
1
33
UNSIGNED BYTE
4096
1.37
4096
1.37
Unaligned multi-frame micrographs of Pichia pastoris (Komagataella phaffii) mitochondrial complex I from an as-prepared sample solubilised in LMNG and reconstituted in to proteoliposomes. Frozen on Qunatifoil gold grids. Gain-corrected mages (MRC) were collected with a 200 keV FEI Talos Arctica on a Falcon 3EC detector. The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 1.69 s exposures were captured in 33 frames with a total dose of 50.37 e-/Å-2. A pixel size of 1.37 Å/pixel was used.