<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-13039" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2024-10-04</depositionDate>
            <releaseDate>2025-10-23</releaseDate>
            <updateDate>2025-10-25</updateDate>
        </keyDates>
        <title>E. coli BAM complex (BamABCDE) in DDM</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-1920-149X</authorORCID>
            <firstName>Jonathan</firstName>
            <middleName>M</middleName>
            <lastName>Machin</lastName>
            <organization type="academic">Faculty of Biological Sciences, University of Leeds</organization>
            <townOrCity>Leeds</townOrCity>
            <stateOrProvince>West Yorkshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>LS2 9JT</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0002-3640-5275</authorORCID>
            <firstName>Neil</firstName>
            <middleName>A</middleName>
            <lastName>Ranson</lastName>
            <organization type="academic">Astbury Centre, University of Leeds</organization>
            <townOrCity>University of Leeds</townOrCity>
            <stateOrProvince>West Yorkshire</stateOrProvince>
            <country>United Kingdom</country>
            <postOrZipCode>LS2 9JT</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-1920-149X">Machin JM</author>
            <author authorORCID="0000-0002-3079-8039">Radford SE</author>
            <author authorORCID="0000-0002-3640-5275">Ranson NA</author>
        </authorsList>
        <grantSupport>
            <grantReference>
                <fundingBody></fundingBody>
                <code></code>
                <country></country>
            </grantReference>
        </grantSupport>
        <datasetSize units="GB">723.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-13039</entryDOI>
        <experimentType>EMDB</experimentType>
        <scale>molecule</scale>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-16282</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Haysom SF</author>
                    <author authorORCID="0000-0002-1920-149X" order="2">Machin J</author>
                    <author order="3">Whitehouse JM</author>
                    <author order="4">Horne JE</author>
                    <author order="5">Fenn K</author>
                    <author authorORCID="0000-0001-5934-0497" order="6">Ma Y</author>
                    <author authorORCID="0000-0002-0552-5784" order="7">El Mkami H</author>
                    <author authorORCID="0009-0000-0673-4820" order="8">Böhringer N</author>
                    <author order="9">Schäberle TF</author>
                    <author order="10">Ranson NA</author>
                    <author authorORCID="0000-0002-3079-8039" order="11">Radford SE</author>
                    <author authorORCID="0000-0002-4309-4858" order="12">Pliotas C</author>
                    <title>Darobactin B Stabilises a Lateral-Closed Conformation of the BAM Complex in E. coli Cells</title>
                    <journal>Angewandte Chemie (International ed. in English)</journal>
                    <journalAbbreviation>Angew Chem Int Ed Engl</journalAbbreviation>
                    <country></country>
                    <issue>34</issue>
                    <volume>62</volume>
                    <year>2023</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1002/anie.202218783</externalReferences>
                    <externalReferences type="pubmed">37162386</externalReferences>
                    <details>Movie stacks are gain corrected but unaligned, 6063 movies, with 47 frames and a total dose per frame of ~0.95 e-/Å2. The total cumulative dose for each movie is ~44.8 e-/Å2 over a ~4 s exposure.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Unaligned movies for E.coli BAM (WT, in DDM) in lateral-open conformation</name>
        <directory>/data</directory>
        <category>micrographs - multiframe</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>6063</numImagesOrTiltSeries>
        <framesPerImage>47</framesPerImage>
        <frameRange>
            <frameRangeMin>1</frameRangeMin>
            <frameRangeMax>47</frameRangeMax>
        </frameRange>
        <voxelType>UNSIGNED BYTE</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>0.74</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>0.74</pixelHeight>
        </dimensions>
        <details>Unaligned movies (47 frames, 44.82 electron/angstrom total dose) collected on falcon 4 (selectris).</details>
        <segmentationList/>
        <micrographsFilePattern>data/Grid*/Data/*tif</micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
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