REL 2016-06-07 2016-06-07 2016-06-07 0000-0003-1248-1828 Yuan Gao Department of Biochemistry and Biophysics, University of California, San Francisco 600 16th Street San Francisco California United States 94158 0000-0001-9535-0369 Yifan Cheng Department of Biochemistry and Biophysics, University of California, San Francisco 600 16th Street San Francisco California United States 94158 0000-0002-6365-4867 David Julius Department of Physiology, University of California, San Francisco 600 16th Street San Francisco California United States 94158 Gao Y 93.8 10.6019/EMPIAR-10059 EMDB EMD-8117 Gao Y Cao E Julius D Cheng Y Nature Nature 7607 534 347 351 2016 English 10.1038/nature17964 27281200 Motion corrected, dose-weighted sum of micrographs of TRPV1-DkTx/RTX embedded in lipid nanodisc /data/micrographs micrographs - single frame MRC MRC 1200 1 UNSIGNED BYTE 3838 1.2156 3710 1.2156

All image stacks (accumulating 41 electrons per squared Angstrom exposure in 30 frames / 6 second exposure) were drift-corrected using software UcsfDfCorr. '5 X 5' patch option was used. Dose rate was set to 1.4 electrons to perform the exposure filtering procedure implemented in the program. Deposited data is the sum of all corrected frames.

Raw particle image stack of TRPV1-DkTx/RTX embedded in lipid nanodisc /data/particles picked particles - multiframe - unprocessed MRC MRC 218805 1 UNSIGNED BYTE 192 1.2156 192 1.2156

Particle image stacks extracted from individual micrographs, without further processing. 'particles.star' list all relevant information for every particle in all image stacks (coordinate, defocus etc.)