REL 2018-02-19 2018-03-21 2022-07-14 Cryo-electron tomography of immature HIV-1 dMACANC VLPs 0000-0003-4790-8078 Florian Schur Institute of Science and Technology Austria Am Campus 1 Klosterneuburg Lower Austria Austria 3400 0000-0003-3990-6910 John AG Briggs MRC Laboratory of Molecular Biology Francis Crick Avenue, Cambridge Biomedical Campus Cambridge Cambridgeshire United Kingdom CB2 0QH Schur FK Obr M Hagen WJ Wan W Jakobi AJ Kirkpatrick JM Sachse C Krausslich HG Briggs JA Turoňová B 865.0 10.6019/EMPIAR-10164 EMDB EMD-3782 EMD-4015 Schur FK Obr M Hagen WJ Wan W Jakobi AJ Kirkpatrick JM Sachse C Kräusslich HG Briggs JA An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation Science (New York, N.Y.) Science 6298 353 506 508 2016 English 10.1126/science.aaf9620 27417497
The data is deposited as raw, compressed frames (8-10 frames). Additional information for each tilt-series can be found in the respective mrc.mdoc files. The subset of data (five tilt series) used for testing in Turonova et al, J Struct Biol. 2017 Sep;199(3):187-195. doi: 10.1016/j.jsb.2017.07.007 are TS_01,TS_03, TS_43, TS_45, TS_54
Turoňová B Schur FKM Wan W Briggs JAG Efficient 3D-CTF correction for cryo-electron tomography using NovaCTF improves subtomogram averaging resolution to 3.4Å Journal of structural biology J. Struct. Biol. 3 199 187 195 2017 English 10.1016/j.jsb.2017.07.007 28743638
The data is deposited as raw, compressed frames (8-10 frames). Additional information for each tilt-series can be found in the respective mrc.mdoc files. The subset of data (five tilt series) used for testing in Turonova et al, J Struct Biol. 2017 Sep;199(3):187-195. doi: 10.1016/j.jsb.2017.07.007 are TS_01,TS_03, TS_43, TS_45, TS_54
 Compressed, unaligned, multi-frame micrographs of tilt series containing HIV-1 dMACANC virus like particles assembled in the presence of BVM. /data/frames micrographs - multiframe MRC MRC 43 8 SIGNED BYTE 7420 1.35 7676 1.35
Information on all tilt series in corresponding .mrc.mdoc files (note that mdoc files refer to binned output, while raw frames are unbinned). Dose per tilt series TS_01 to TS_16: 3.0 e/A2/sec TS_17 to TS_40: 3.5 e/A2/sec TS_41 to TS_50: 3.1 e/A2/sec TS_51 to TS_63: 3.0 e/A2/sec The frames are compressed, without any alignment, and gain corrected. The subset of data (5 tomograms) that was used for testing in Turonova et al, J Struct Biol. 2017 Sep;199(3):187-195. doi: 10.1016/j.jsb.2017.07.007 is TS_01 TS_03 TS_43 TS_45 TS_54 Part of the dataset is 8 frames per image, part of the dataset is 10 frames per image. All frames were used.