REL
2018-10-05
2018-10-25
2019-04-10
CryoET of bacterial RNA polymerase with several detergents
0000-0002-8241-3153
Darst
Seth
The Rockefeller University
1230 York Avenue
New York
NY
United States
10065
0000-0002-8241-3153
Darst
Seth
The Rockefeller University
1230 York Avenue
New York
NY
United States
10065
Chen J
Noble AJ
Kang JY
Darst SA
207.5
10.6019/EMPIAR-10214
EMDB
Chen J
Noble AJ
Kang JY
Darst SA
Eliminating effects of particle adsorption to the air/water interface in single-particle cryo-electron microscopy: Bacterial RNA polymerase and CHAPSO
Journal of Structural Biology: X
2019
English
10.1016/j.yjsbx.2019.100005
The tilt images are whole-frame aligned averages using Motioncor2.
K2 tilt-series frames
/data/frames
micrographs - multiframe
MRCS
MRC
8
8
UNSIGNED 16 BIT INTEGER
3710
1.33
3838
1.33
~8 e-/px/s dose rate on the camera
Gain and dark references included
Whole-frame aligned tilt images along with all other magnification images from the collection
/data/leginon
micrographs - single frame
MRC
MRC
17
1
32 BIT FLOAT
3710
1.33
3838
1.33
Whole-frame aligned tilt images using Motioncor2.
Dynamo manual particle picks of tomograms
/data/particle picks
picked particles - multiframe - unprocessed
DYNAMO TBL FILE
RAW TEXT
4
1
32 BIT FLOAT
variable
5.32
variable
5.32
Manual particle picks of binned by 4 tomograms using Dynamo.
Appion-Protomo tilt-series alignments
/data/protomo_alignments
tilt series
MRC
MRC
13
1
32 BIT FLOAT
3710
1.33
3838
1.33
Includes a full tilt-series alignment run for each tilt-series.
To view properly, navigate to the protomo_alignments folder in Appion-Protomo.
The export/ directory contains SerialEM-formatted unaligned tilt-series stacks, mdoc files, and tilt files. This allows for easy upload to Appion-Protomo.