FAK APO DATASET - apo/
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Paticle stack - apo/particles/
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particles_preprocessed.mrcs - stack file containing 361,796 particles extracted from the 2D crystal images with FOCUS, 238 x 238 pixels, 1.7 A/px (Polara + Titan datasets merged)
particles_preprocessed.star - metadata file for 361,796 particles as extracted from the 2D crystal images with FOCUS

APO/Polara - apo/micrographs/polara/
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Acceleration voltage = 300 kV
Cs = 2.26 mm
Nominal pixel size = 1.7 A/px
Total exposure = 40 e- / A^2 distributed over 50 frames for 10 s
Detector = Gatan K2

Data structure:

apo/micrographs/polara/alignaver/ : dose-weighted aligned averages from MotionCor2
apo/micrographs/polara/config/ : processing metadata from FOCUS including tilt geometry, defocus, etc.
apo/micrographs/polara/coordinates/ : coordinates of particles picked from each micrograph in .box format

NOTE: All filenames contain an unique ID and their nominal tilt angles.

APO/Titan - apo/micrographs/titan/
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Acceleration voltage = 300 kV
Cs = 2.7 mm
Phyisical pixel size = 0.529 A/px
Super-resolution pixel size = 1.058 A/px
Total exposure = 40 e- / A^2 distributed over 80 frames for 10 s
Detector = Gatan K2 with Quantum-LS energy filter (20 slit eV width)

Data structure:

apo/micrographs/titan/alignaver/ : dose-weighted aligned averages from MotionCor2
apo/micrographs/titan/config/ : processing metadata from FOCUS including tilt geometry, defocus, etc.
apo/micrographs/titan/coordinates/ : coordinates of particles picked from each micrograph in .box format
apo/micrographs/titan/gainref/ : gain reference in DM4 format
apo/micrographs/titan/raw/ : raw movies in TIFF format

NOTE: All filenames within the dataset contain an unique FOCUS ID and their nominal tilt angles.


FAK AMP-PNP DATASET - amp-pnp/
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Paticle stack - amp-pnp/particles/
**********************************

particles.mrcs - stack file containing 2,666,154 particles extracted from the 2D crystal images with FOCUS, 200 x 200 pixels, 2.116 A/px
particles.star - metadata file for 2,666,154 particles as extracted from the 2D crystal images with FOCUS

AMP-PNP/Titan - amp-pnp/micrographs/
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Acceleration voltage = 300 kV
Cs = 2.7 mm
Phyisical pixel size = 0.529 A/px
Super-resolution pixel size = 1.058 A/px
Total exposure = 80 e- / A^2 distributed over 40 frames for 8 s
Detector = Gatan K2 with Quantum-LS energy filter (20 slit eV width)

Data structure:

amp-pnp/micrographs/titan/alignaver/ : dose-weighted aligned averages from MotionCor2
amp-pnp/micrographs/titan/config/ : processing metadata from FOCUS including tilt geometry, defocus, etc.
amp-pnp/micrographs/titan/coordinates/ : coordinates of particles picked from each micrograph in .box format
amp-pnp/micrographs/titan/gainref/ : gain reference in DM4 format
amp-pnp/micrographs/titan/raw/ : raw movies in TIFF format

NOTE: All filenames within the dataset contain an unique FOCUS ID and their measured tilt angles.