REL
2020-02-10
2020-07-01
2022-01-14
Single particle cryo electron microscopy of the human serotonin transporter bound with paroxetine and in complex with 8B6 Fab
0000-0003-0001-6195
Jonathan
Coleman
Vollum Institute, Oregon Health & Science University
3232 SW Research Dr
Portland
Oregon
United States
97239
0000-0002-8549-2360
Eric
Gouaux
Vollum Institute, Oregon Health & Science University
3232 SW Research Dr
Portland
Oregon
United States
97239
Coleman JA
Navratna V
Antermite D
Yang D
Bull JA
Gouaux E
2.7
10.6019/EMPIAR-10380
EMDB
EMD-21368
Coleman JA
Navratna V
Antermite D
Yang D
Bull JA
Gouaux E
Chemical and structural investigation of the paroxetine-human serotonin transporter complex
eLife
Elife
9
2020
English
10.7554/elife.56427
32618269
Dataset 1 unaligned multi-frame micrographs of SERT-8B6 Fab complex bound to paroxetine
/data/unaligned_micrographs_1
micrographs - multiframe
TIFF
TIFF
2557
40
1
40
UNSIGNED BYTE
11520
0.324
8184
0.324
Multiframe movies contain 40 raw frames with a total dose of 54 e-/A^2.
data/unaligned_micrographs_1/F_g8_*.tif
data/coord/F_g8_*_mc_DW_coord.star
data/coord
Dataset 2 unaligned multi-frame micrographs of SERT-8B6 Fab complex bound to paroxetine
/data/unaligned_micrographs_2
micrographs - multiframe
TIFF
TIFF
4613
40
1
40
UNSIGNED BYTE
11520
0.324
8184
0.324
Multiframe movies contain 40 raw frames with a total dose of 60 e-/A^2.
data/unaligned_micrographs_2/g3_F_paro_*.tif
data/coord/g3_F_paro_*_mc_DW_coord.star
data/coord
Aligned micrographs of the SERT-8B6 paroxetine Fab complex bound to paroxetine
/data/aligned_micrographs
micrographs - single frame
MRC
MRC
7170
1
32 BIT FLOAT
5760
0.648
4092
0.648
Aligned micrographs. Picked particles also follow pattern: data/coord/g3_F_paro_05702_mc_DW_coord.star
data/aligned_micrographs/F_g8_00000_mc_DW.mrc
data/coord/F_g8_00000_mc_DW_coord.star
data/coord
Particle stack of the SERT-8B6 paroxetine Fab complex bound to paroxetine
/data/picked_particles
picked particles - multiframe - unprocessed
MRC
MRC
420373
1
32 BIT FLOAT
400
0.648
400
0.648
Final refined particle stack and coordinates. Picked particles also follow pattern: data/coord/g3_F_paro_05702_mc_DW_coord.star
data/aligned_micrographs/F_g8_00000_mc_DW.mrc
data/coord/F_g8_*_mc_DW_coord.star
data/coord