Y. enterocolitica Urease dataset ******************************** Acceleration voltage = 300 kV Cs = 2.7 mm Physical pixel size = 0.639 A/px Total exposure = 42 e- / A^2 distributed over 40 frames for 8 s Detector = Gatan K2 with Quantum-LS energy filter (20 slit eV width) Data structure: alignaver/ : This directory contains dose-weighted aligned movie averages from MotionCor2, the coordinates of the Gautomatch picked particles (STAR and BOX formats), and the meta-data for the Bayesian polishing (STAR format). gainref/ : This directory contains the gain references in DM4 and MRC formats, pixel defect lists in TXT format. raw/ : raw movies in TIFF format micrographs_multi+single_joined.star : this file contains all the micrographs used for processing with their respective _rlnBeamTiltClass label. run_ct14_data.star : this file contains the metadata for the particles used in the final reconstruction. NOTE: The directories contain all data from the multi- and single-shot movies together, as specified by the file name. ***Update from 14/12/2020*** Dataset B-factor ---------------- Information on the Rosenthal/Henderson a.k.a. "ResLog" plot with C1 and T symmetries has been added for this dataset (bfactor_urease.png). Nominal resolution (FSC 0.143, T symmetry, all particles): 1.98 Å Sharpening B-factor (estimated from Guinier plot): -38 Å^2 Reconstruction ("ResLog") B-factor: 64.1 Å^2 (C1) and 71.5 Å^2 (T)