<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10404" schemaVersion="0.65" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2020-04-30</depositionDate>
            <releaseDate>2020-05-01</releaseDate>
            <updateDate>2021-06-18</updateDate>
        </keyDates>
        <title>SARS-CoV-2 productively infects human gut enterocytes</title>
        <correspondingAuthor private="true">
            <authorORCID>0000-0002-7539-1160</authorORCID>
            <firstName>Kèvin</firstName>
            <lastName>Knoops</lastName>
            <organization type="academic">Maastricht University - M4I</organization>
            <street>Universiteitssingel 50</street>
            <townOrCity>Maastricht</townOrCity>
            <country>Netherlands</country>
            <postOrZipCode>6229 ER</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-6591-4637</authorORCID>
            <firstName>Hans</firstName>
            <lastName>Clevers</lastName>
            <organization type="academic">Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center</organization>
            <street>Uppsalalaan 8</street>
            <townOrCity>Utrecht</townOrCity>
            <country>Netherlands</country>
            <postOrZipCode>3584 CT</postOrZipCode>
        </principalInvestigator>
        <principalInvestigator private="true">
            <authorORCID>0000-0001-6221-2015</authorORCID>
            <firstName>Bart</firstName>
            <lastName>Haagmans</lastName>
            <organization type="academic">Viroscience Department, Erasmus Medical Center, Rotterdam, Netherlands</organization>
            <street>Dr. Molewaterplein 40</street>
            <townOrCity>Rotterdam</townOrCity>
            <country>Netherlands</country>
            <postOrZipCode>3015 GD</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author authorORCID="0000-0002-1431-4022">Lamers MM</author>
            <author authorORCID="0000-0002-2055-4444">Beumer J</author>
            <author authorORCID="0000-0002-4126-8420">van der Vaart J</author>
            <author authorORCID="0000-0002-7539-1160">Knoops K</author>
            <author>Puschhof J</author>
            <author>Breugem T</author>
            <author authorORCID="0000-0001-6056-5888">Ravelli RBG</author>
            <author authorORCID="0000-0001-6591-4637">van Schayck JP</author>
            <author>Mykytyn AZ</author>
            <author>Duimel HQ</author>
            <author>van Donselaar E</author>
            <author>Riesebosch S</author>
            <author>Kuijpers HJH</author>
            <author>Schipper D</author>
            <author>van de Wetering WJ</author>
            <author>de Graaf M</author>
            <author authorORCID="0000-0002-5204-2312">Koopmans M</author>
            <author authorORCID="0000-0002-0400-9542">Cuppen E</author>
            <author>Peters PJ</author>
            <author authorORCID="0000-0001-6221-2015">Haagmans B</author>
            <author authorORCID="0000-0002-3077-5582">Clevers H</author>
        </authorsList>
        <datasetSize units="GB">156.2</datasetSize>
        <entryDOI>10.6019/EMPIAR-10404</entryDOI>
        <experimentType>Exception</experimentType>
    </admin>
    <crossReferences>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author authorORCID="0000-0002-1431-4022" order="1">Lamers MM</author>
                    <author authorORCID="0000-0002-2055-4444" order="2">Beumer J</author>
                    <author authorORCID="0000-0002-4126-8420" order="3">van der Vaart J</author>
                    <author authorORCID="0000-0002-7539-1160" order="4">Knoops K</author>
                    <author order="5">Puschhof J</author>
                    <author order="6">Breugem T</author>
                    <author authorORCID="0000-0001-6056-5888" order="7">Ravelli RBG</author>
                    <author authorORCID="0000-0001-6591-4637" order="8">van Schayck JP</author>
                    <author order="9">Mykytyn AZ</author>
                    <author order="10">Duimel HQ</author>
                    <author order="11">van Donselaar E</author>
                    <author order="12">Riesebosch S</author>
                    <author order="13">Kuijpers HJH</author>
                    <author order="14">Schipper D</author>
                    <author order="15">van de Wetering WJ</author>
                    <author order="16">de Graaf M</author>
                    <author authorORCID="0000-0002-5204-2312" order="17">Koopmans M</author>
                    <author authorORCID="0000-0002-0400-9542" order="18">Cuppen E</author>
                    <author order="19">Peters PJ</author>
                    <author authorORCID="0000-0001-6221-2015" order="20">Haagmans B</author>
                    <author authorORCID="0000-0002-3077-5582" order="21">Clevers H</author>
                    <title>SARS-CoV-2 productively infects human gut enterocytes</title>
                    <journal>Science</journal>
                    <journalAbbreviation>Science</journalAbbreviation>
                    <country>United States</country>
                    <externalReferences type="doi">10.1126/science.abc1669</externalReferences>
                    <externalReferences type="pubmed">32358202</externalReferences>
                    <details>These are the complete stitched maps in OME-TIFF pyramidal format (BioFormats 6.0 or higher required for reading files) and the raw unstitched tiffs.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
        <relatedIdrEntries>
            <idrEntry>idr0083</idrEntry>
        </relatedIdrEntries>
    </crossReferences>
    <imageSet>
        <name>12448_G1HR - TEM analysis of SARS-CoV-2 infected intestine organoids</name>
        <directory>/data</directory>
        <category>stitched maps</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>144384</imageWidth>
            <pixelWidth>10.0</pixelWidth>
            <imageHeight>93184</imageHeight>
            <pixelHeight>10.0</pixelHeight>
        </dimensions>
        <details>Stitched map of TEM analysis of SARS-CoV-2 infected intestine organoids. Stitched map in OME-TIFF pyramidal format (BioFormats 6.0 or higher required for reading file).

The data can be examined in IDR:
&lt;a href="https://idr.openmicroscopy.org/search/?query=Name:idr0083"&gt;https://idr.openmicroscopy.org/search/?query=Name:idr0083&lt;/a&gt;
and 
&lt;a href="https://idr.openmicroscopy.org/webclient/?show=project-1051"&gt;https://idr.openmicroscopy.org/webclient/?show=project-1051&lt;/a&gt;

Direct links to images:
&lt;a href="https://idr.openmicroscopy.org/study/idr0083/figure/4i"&gt;https://idr.openmicroscopy.org/study/idr0083/figure/4i&lt;/a&gt;
&lt;a href="https://idr.openmicroscopy.org/study/idr0083/figure/4r"&gt;https://idr.openmicroscopy.org/study/idr0083/figure/4r&lt;/a&gt;</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>12448_J1HR - TEM analysis of SARS-CoV-2 infected intestine organoids</name>
        <directory>/data</directory>
        <category>stitched maps</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>79360</imageWidth>
            <pixelWidth>10.0</pixelWidth>
            <imageHeight>167424</imageHeight>
            <pixelHeight>10.0</pixelHeight>
        </dimensions>
        <details>Stitched map of TEM analysis of SARS-CoV-2 infected intestine organoids. Stitched map in OME-TIFF pyramidal format (BioFormats 6.0 or higher required for reading file).

The data can be examined in IDR:
&lt;a href="https://idr.openmicroscopy.org/search/?query=Name:idr0083"&gt;https://idr.openmicroscopy.org/search/?query=Name:idr0083&lt;/a&gt;
and 
&lt;a href="https://idr.openmicroscopy.org/webclient/?show=project-1051"&gt;https://idr.openmicroscopy.org/webclient/?show=project-1051&lt;/a&gt;

Direct links to images:
&lt;a href="https://idr.openmicroscopy.org/study/idr0083/figure/4i"&gt;https://idr.openmicroscopy.org/study/idr0083/figure/4i&lt;/a&gt;
&lt;a href="https://idr.openmicroscopy.org/study/idr0083/figure/4r"&gt;https://idr.openmicroscopy.org/study/idr0083/figure/4r&lt;/a&gt;</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>12448_G1HR - Raw micrographs used to stitch 12448_G1HR_Mesh003.ome.tif</name>
        <directory>/data/12448_G1HR/Mesh003</directory>
        <category>micrographs - single frame</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1470</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>10.0</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>10.0</pixelHeight>
        </dimensions>
        <details>All raw micrographs used for stitching. Also contains the stage coordinates of each micrograph in xml and matlab format. For each file a log file is available with the settings used.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
    <imageSet>
        <name>12448_J1HR - Raw micrographs used to stitch 12448_J1HR_Mesh001.ome.tif</name>
        <directory>/data/12448_J1HR/Mesh001</directory>
        <category>micrographs - single frame</category>
        <headerFormat>TIFF</headerFormat>
        <dataFormat>TIFF</dataFormat>
        <numImagesOrTiltSeries>1400</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>UNSIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>4096</imageWidth>
            <pixelWidth>10.0</pixelWidth>
            <imageHeight>4096</imageHeight>
            <pixelHeight>10.0</pixelHeight>
        </dimensions>
        <details>All raw micrographs used for stitching. Also contains the stage coordinates of each micrograph in xml and matlab format. For each file a log file is available with the settings used.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
