<entry xmlns="http://pdbe.org/empiar" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://ftp.ebi.ac.uk/pub/databases/emtest/empiar/schema/empiar.xsd" accessionCode="EMPIAR-10694" public="true">
    <admin>
        <currentStatus>REL</currentStatus>
        <keyDates>
            <depositionDate>2021-04-13</depositionDate>
            <releaseDate>2021-04-14</releaseDate>
            <updateDate>2021-04-14</updateDate>
        </keyDates>
        <title>In situ cryo-electron tomogram of a pyrenoid inside a Chlamydomonas reinhardtii cell (tilt series)</title>
        <correspondingAuthor>
            <authorORCID>0000-0003-4247-4303</authorORCID>
            <firstName>Ricardo</firstName>
            <middleName>D.</middleName>
            <lastName>Righetto</lastName>
            <organization type="academic">Helmholtz Zentrum München</organization>
            <townOrCity>Munich</townOrCity>
            <country>Germany</country>
            <postOrZipCode>85764</postOrZipCode>
        </correspondingAuthor>
        <principalInvestigator>
            <authorORCID>0000-0002-0941-4387</authorORCID>
            <firstName>Benjamin</firstName>
            <middleName>D.</middleName>
            <lastName>Engel</lastName>
            <organization type="academic">Helmholtz Pioneer Campus</organization>
            <townOrCity>Munich</townOrCity>
            <country>Germany</country>
            <postOrZipCode>85764</postOrZipCode>
        </principalInvestigator>
        <authorsList>
            <author>Cuellar LK</author>
            <author>Schaffer M</author>
            <author>Martinez-Sanchez A</author>
            <author>Plitzko JM</author>
            <author>Foerster F</author>
            <author>Engel BD</author>
        </authorsList>
        <datasetSize>5.8</datasetSize>
        <entryDOI>10.6019/EMPIAR-10694</entryDOI>
        <experimentType>EMDB</experimentType>
    </admin>
    <crossReferences>
        <relatedEMDBEntries>
            <emdbEntry>EMD-12749</emdbEntry>
        </relatedEMDBEntries>
        <citationList>
            <universalCitation>
                <journalCitation published="true" preprint="false">
                    <author order="1">Freeman Rosenzweig ES</author>
                    <author order="2">Xu B</author>
                    <author order="3">Kuhn Cuellar L</author>
                    <author order="4">Martinez-Sanchez A</author>
                    <author authorORCID="0000-0001-8748-1748" order="5">Schaffer M</author>
                    <author authorORCID="0000-0002-0986-2868" order="6">Strauss M</author>
                    <author authorORCID="0000-0001-6639-5808" order="7">Cartwright HN</author>
                    <author authorORCID="0000-0002-9012-7544" order="8">Ronceray P</author>
                    <author authorORCID="0000-0002-6402-8315" order="9">Plitzko JM</author>
                    <author order="10">Förster F</author>
                    <author order="11">Wingreen NS</author>
                    <author authorORCID="0000-0002-0941-4387" order="12">Engel BD</author>
                    <author order="13">Mackinder LCM</author>
                    <author order="14">Jonikas MC</author>
                    <title>The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization</title>
                    <journal>Cell</journal>
                    <journalAbbreviation>Cell</journalAbbreviation>
                    <country></country>
                    <issue>1</issue>
                    <volume>171</volume>
                    <firstPage>148</firstPage>
                    <lastPage>162.e19</lastPage>
                    <year>2017</year>
                    <language>English</language>
                    <externalReferences type="doi">10.1016/j.cell.2017.08.008</externalReferences>
                    <externalReferences type="pubmed">28938114</externalReferences>
                    <details>Tilt series and metadata for tomogram reconstruction.</details>
                </journalCitation>
            </universalCitation>
        </citationList>
    </crossReferences>
    <imageSet>
        <name>Phase-flipped and aligned tilt series of a pyrenoid inside a Chlamydomonas reinhardtii cell</name>
        <directory>/data</directory>
        <category>tilt series</category>
        <headerFormat>MRC</headerFormat>
        <dataFormat>MRC</dataFormat>
        <numImagesOrTiltSeries>1</numImagesOrTiltSeries>
        <framesPerImage>1</framesPerImage>
        <voxelType>SIGNED 16 BIT INTEGER</voxelType>
        <dimensions>
            <imageWidth>3712</imageWidth>
            <pixelWidth>3.42</pixelWidth>
            <imageHeight>3712</imageHeight>
            <pixelHeight>3.42</pixelHeight>
        </dimensions>
        <details>L3Tomo3.st is the original unbinned tilt series after motion correction and dose-weighting of individual tilts.
L3Tomo3.st is the aligned and phase-flipped unbinned tilt series for tomogram reconstruction.
L3Tomo3_bin4.rec is the bin4 tomogram for IMOD consistency.
Other files are accessory for tomogram reconstruction and subtomogram extraction. See README.txt for details.</details>
        <segmentationList/>
        <micrographsFilePattern></micrographsFilePattern>
        <pickedParticlesFilePattern></pickedParticlesFilePattern>
        <pickedParticlesDirectory></pickedParticlesDirectory>
    </imageSet>
</entry>
