REL
2021-08-23
2021-11-10
2021-11-10
Structural mechanism of GTPase-powered ribosome-tRNA movement
0000-0002-4609-4052
Niels
Fischer
Department of Structural Dynamics, Max Planck Institute for Biophysical Chemistry
Am Fassberg 11
Göttingen
Lower Saxony
Germany
37077
0000-0002-4609-4052
Niels
Fischer
Department of Structural Dynamics, Max Planck Institute for Biophysical Chemistry
Am Fassberg 11
Göttingen
Lower Saxony
Germany
37077
0000-0003-0105-3879
Marina
V.
Rodnina
Department of Physical Biochemistry, Max Planck Institute for Biophysical Chemistry
Am Fassberg 11
Göttingen
Lower Saxony
Germany
37077
Petrychenko V
Peng B-Z
Schwarzer AP
Peske F
Rodnina MV
Fischer N
987.5
10.6019/EMPIAR-10792
EMDB
EMD-13458
EMD-13459
EMD-13460
EMD-13461
EMD-13462
EMD-13463
EMD-13464
EMD-13465
Petrychenko V
Peng BZ
de A P Schwarzer AC
Peske F
Rodnina MV
Fischer N
Structural mechanism of GTPase-powered ribosome-tRNA movement
Nature communications
Nat Commun
1
12
2021
English
10.1038/s41467-021-26133-x
34635670
Motion-corrected, dose-weighted micrographs
/data/data/corrected_micrographs
micrographs - single frame
MRC
MRC
9300
1
32 BIT FLOAT
4096
1.16
4096
1.16
Note: Micrographs were obtained from movie-stacks of 20 frames à 1.5 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion.
- Micrographs .star-file: "corrected_micrographs.star"
data/data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc
Shiny particles of non-rotated ribosome particles (C state), obtained by Bayesian polishing in Relion 3.1
/data/data/shiny_Cstate
picked particles - single frame - processed
MRCS
MRCS
1176423
1
32 BIT FLOAT
288
1.16
288
1.16
Shiny particles .star-file & 3D mask for final 3D refinement of C state (EMD-13458): "shiny_Cstate.star" & "mask_Cstate.mrc"
data/data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc
data/data/shiny_Cstate/FoilHole_*_Data_*_Fractions_shiny.mrcs
data/data/shiny_Cstate
Shiny particles of rotated ribosome particles, obtained by Bayesian polishing in Relion 3.1
/data/data/shiny
picked particles - single frame - processed
MRCS
MRCS
103222
1
32 BIT FLOAT
288
1.16
288
1.16
Star-files of shiny particles & 3D masks used for final 3D refinements:
- H1 (EMD-13459): "shiny_H1.star" & "mask_H1.mrc"
- H2 (EMD-13460): "shiny_H2.star" & "mask_H2.mrc"
- H1-EF-G-GDP-Pi (EMD-13461): "shiny_H1-EF-G-GDP-Pi.star" & "mask_H1-EF-G-GDP-Pi.mrc"
- H2-EF-G-GDP-Pi (EMD-13462): "shiny_H2-EF-G-GDP-Pi.star" & "mask_H2-EF-G-GDP-Pi.mrc"
- H1-EF-G-GDP (EMD-13463): "shiny_H1-EF-G-GDP.star" & "mask_H1-EF-G-GDP.mrc"
- CHI1-EF-G-GDP (EMD-13464): "shiny_CHI1-EF-G-GDP.star" & "mask_CHI1-EF-G-GDP.mrc"
- CHI2-EF-G-GDP (EMD-13465): "shiny_CHI2-EF-G-GDP.star" & "mask_CHI2-EF-G-GDP.mrc"
Star-files of shiny particles for substates 1-3 of EF-G domain 4 in H1-EF-G-GDP-Pi state (EMD-13461):
- "shiny_H1-EF-G-GDP-Pi_D4_substate1.star"
- "shiny_H1-EF-G-GDP-Pi_D4_substate2.star"
- "shiny_H1-EF-G-GDP-Pi_D4_substate3.star"
data/data/corrected_micrographs/FoilHole_*_Data_*_Fractions.mrc
data/data/shiny/FoilHole_*_Data_*_Fractions_shiny.mrcs
data/data/shiny