>> HOW TO MAKE TUTORIAL DATA OF EMPIAR-10248 << 2019/08/23 Takeshi Kawabata EMPIAR-10248 : 971 TIFF files. (1) For the 971 TIFF files, we execulted MotironCorr and CTFfind using Relion 3.0. The file "micrographs_ctf.star" was generated after the CTFfind calculation. (2) Among the 971 TIFF files of EMPIAR-10248 with_rlnCtfFigureOfMerit > 0.1, the 20 TIFF files with the best _rlnCtfMaxResolution have been taken. $ sort -n -k 13 micrographs_ctf.star | awk '(NF>10)&&($12>0.1){print $1,$12,$13}' |head -20 MotionCorr/job002/Movies//190117_02_00098_UnderDefocus1_2um_frameImage.mrc 0.138845 1.878000 MotionCorr/job002/Movies//190117_02_00431_UnderDefocus0_9um_frameImage.mrc 0.176702 1.977000 MotionCorr/job002/Movies//190117_02_00823_UnderDefocus1_1um_frameImage.mrc 0.161487 2.217000 MotionCorr/job002/Movies//190117_02_00362_UnderDefocus0_7um_frameImage.mrc 0.170195 2.222000 MotionCorr/job002/Movies//190117_02_00081_UnderDefocus0_6um_frameImage.mrc 0.183014 2.224000 MotionCorr/job002/Movies//190117_02_00945_UnderDefocus0_8um_frameImage.mrc 0.153353 2.239000 MotionCorr/job002/Movies//190117_02_00282_UnderDefocus0_7um_frameImage.mrc 0.171375 2.249000 MotionCorr/job002/Movies//190117_02_00150_UnderDefocus0_5um_frameImage.mrc 0.175096 2.329000 MotionCorr/job002/Movies//190117_02_00927_UnderDefocus0_6um_frameImage.mrc 0.182210 2.334000 MotionCorr/job002/Movies//190117_02_00468_UnderDefocus0_6um_frameImage.mrc 0.221338 2.337000 MotionCorr/job002/Movies//190117_02_00809_UnderDefocus1_1um_frameImage.mrc 0.143523 2.337000 MotionCorr/job002/Movies//190117_02_00730_UnderDefocus0_9um_frameImage.mrc 0.158823 2.386000 MotionCorr/job002/Movies//190117_02_00797_UnderDefocus0_9um_frameImage.mrc 0.169620 2.386000 MotionCorr/job002/Movies//190117_02_00441_UnderDefocus0_9um_frameImage.mrc 0.159107 2.555000 MotionCorr/job002/Movies//190117_02_00120_UnderDefocus1_0um_frameImage.mrc 0.226052 2.561000 MotionCorr/job002/Movies//190117_02_00436_UnderDefocus0_9um_frameImage.mrc 0.172798 2.574000 MotionCorr/job002/Movies//190117_02_00557_UnderDefocus1_2um_frameImage.mrc 0.169714 2.614000 MotionCorr/job002/Movies//190117_02_00770_UnderDefocus0_5um_frameImage.mrc 0.245673 2.617000 MotionCorr/job002/Movies//190117_02_00967_UnderDefocus0_7um_frameImage.mrc 0.168465 2.648000 MotionCorr/job002/Movies//190117_02_00277_UnderDefocus0_7um_frameImage.mrc 0.235441 2.676000 (3) Each TIFF file has 50 frames. We took only first 25 frames. Then number of image frames becomes almost 1/100 ( 20/971 /2 = .01029866). However, using this reduced dataset, we will get a 3D map with almost 3.0 angstrom resolution.