RELION version: 3.1-beta-commit-6c49f8 Precision: BASE=double, CUDA-ACC=single === RELION MPI setup === + Number of MPI processes = 3 + Number of threads per MPI process = 2 + Total number of threads therefore = 6 + Master (0) runs on host = embox + Slave 1 runs on host = embox + Slave 2 runs on host = embox ================= uniqueHost embox has 2 ranks. Slave 1 will distribute threads over devices 0 Thread 0 on slave 1 mapped to device 0 Thread 1 on slave 1 mapped to device 0 Slave 2 will distribute threads over devices 1 Thread 0 on slave 2 mapped to device 1 Thread 1 on slave 2 mapped to device 1 Running CPU instructions in double precision. Estimating initial noise spectra 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 2 sec ....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec ..........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Iteration= 1 Auto-refine: Resolution= 27.9168 (no gain for 0 iter) Auto-refine: Changes in angles= 999 degrees; and in offsets= 999 Angstroms (no gain for 0 iter) CurrentResolution= 27.9168 Angstroms, which requires orientationSampling of at least 22.5 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 3528 OrientationalSampling= 15 NrOrientations= 168 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 112896 OrientationalSampling= 7.5 NrOrientations= 1344 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 1 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 0 sec ..............................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 7/ 7 sec ............................................................~~(,_,"> Auto-refine: Iteration= 2 Auto-refine: Resolution= 27.9168 (no gain for 1 iter) Auto-refine: Changes in angles= 22.3319 degrees; and in offsets= 1.21851 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ...................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec ......................................~~(,_,"> 0/ 0 sec .......................................~~(,_,"> 0/ 0 sec .......................................~~(,_,"> 0/ 0 sec ........................................~~(,_,"> 0/ 0 sec ........................................~~(,_,"> 0/ 0 sec .........................................~~(,_,"> 0/ 0 sec ..........................................~~(,_,"> 0/ 0 sec ..........................................~~(,_,"> 0/ 0 sec ...........................................~~(,_,"> 0/ 0 sec ...........................................~~(,_,"> 0/ 0 sec ............................................~~(,_,"> 0/ 0 sec .............................................~~(,_,"> 0/ 0 sec .............................................~~(,_,"> 0/ 0 sec ..............................................~~(,_,"> 0/ 0 sec ..............................................~~(,_,"> 0/ 0 sec ...............................................~~(,_,"> 0/ 0 sec ................................................~~(,_,"> 0/ 0 sec ................................................~~(,_,"> 0/ 0 sec .................................................~~(,_,"> 0/ 0 sec .................................................~~(,_,"> 0/ 0 sec ..................................................~~(,_,"> 0/ 0 sec ...................................................~~(,_,"> 0/ 0 sec ...................................................~~(,_,"> 0/ 0 sec ....................................................~~(,_,"> 0/ 0 sec ....................................................~~(,_,"> 0/ 0 sec .....................................................~~(,_,"> 0/ 0 sec ......................................................~~(,_,"> 0/ 0 sec ......................................................~~(,_,"> 0/ 0 sec .......................................................~~(,_,"> 0/ 0 sec .......................................................~~(,_,"> 0/ 0 sec ........................................................~~(,_,"> 0/ 0 sec .........................................................~~(,_,"> 0/ 0 sec .........................................................~~(,_,"> 0/ 0 sec ..........................................................~~(,_,"> 0/ 0 sec ..........................................................~~(,_,"> 0/ 0 sec ...........................................................~~(,_,"> 0/ 0 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 14.86 degrees; offsets= 2.70455 Angstroms Auto-refine: WARNING: The angular accuracy is worse than 10 degrees, so basically you cannot align your particles (yet)! Auto-refine: WARNING: You probably need not worry if the accuracy improves during the next few iterations. Auto-refine: WARNING: However, if the problem persists it may lead to spurious FSC curves, so be wary of inflated resolution estimates... Auto-refine: WARNING: Sometimes it is better to tune resolution yourself by adjusting T in a 3D-classification with a single class. Auto-refine: Angular step= 7.5 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 27.9168 Angstroms, which requires orientationSampling of at least 22.5 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 3528 OrientationalSampling= 15 NrOrientations= 168 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 112896 OrientationalSampling= 7.5 NrOrientations= 1344 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 2 000/??? sec ~~(,_,"> [oo] 1/ 6 sec .........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 2/ 3 sec ..................................~~(,_,"> 2/ 3 sec .....................................~~(,_,"> 2/ 2 sec ........................................~~(,_,"> 2/ 2 sec ...........................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 3/ 2 sec ..............................................................~~(,_,"> 3/ 3 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 7/ 7 sec ............................................................~~(,_,"> Auto-refine: Iteration= 3 Auto-refine: Resolution= 9.97029 (no gain for 0 iter) Auto-refine: Changes in angles= 13.7565 degrees; and in offsets= 0.971179 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ...................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec ......................................~~(,_,"> 0/ 0 sec .......................................~~(,_,"> 0/ 0 sec .......................................~~(,_,"> 0/ 0 sec ........................................~~(,_,"> 0/ 0 sec ........................................~~(,_,"> 0/ 0 sec .........................................~~(,_,"> 0/ 0 sec ..........................................~~(,_,"> 0/ 0 sec ..........................................~~(,_,"> 0/ 0 sec ...........................................~~(,_,"> 0/ 0 sec ...........................................~~(,_,"> 0/ 0 sec ............................................~~(,_,"> 0/ 0 sec .............................................~~(,_,"> 0/ 0 sec .............................................~~(,_,"> 0/ 0 sec ..............................................~~(,_,"> 0/ 0 sec ..............................................~~(,_,"> 0/ 0 sec ...............................................~~(,_,"> 0/ 0 sec ................................................~~(,_,"> 0/ 0 sec ................................................~~(,_,"> 0/ 0 sec .................................................~~(,_,"> 0/ 0 sec .................................................~~(,_,"> 0/ 0 sec ..................................................~~(,_,"> 0/ 0 sec ...................................................~~(,_,"> 0/ 0 sec ...................................................~~(,_,"> 0/ 0 sec ....................................................~~(,_,"> 0/ 0 sec ....................................................~~(,_,"> 0/ 0 sec .....................................................~~(,_,"> 0/ 0 sec ......................................................~~(,_,"> 0/ 0 sec ......................................................~~(,_,"> 0/ 0 sec .......................................................~~(,_,"> 0/ 0 sec .......................................................~~(,_,"> 0/ 0 sec ........................................................~~(,_,"> 0/ 0 sec .........................................................~~(,_,"> 0/ 0 sec .........................................................~~(,_,"> 0/ 0 sec ..........................................................~~(,_,"> 0/ 0 sec ..........................................................~~(,_,"> 0/ 0 sec ...........................................................~~(,_,"> 0/ 0 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 14.44 degrees; offsets= 2.3474 Angstroms Auto-refine: WARNING: The angular accuracy is worse than 10 degrees, so basically you cannot align your particles (yet)! Auto-refine: WARNING: You probably need not worry if the accuracy improves during the next few iterations. Auto-refine: WARNING: However, if the problem persists it may lead to spurious FSC curves, so be wary of inflated resolution estimates... Auto-refine: WARNING: Sometimes it is better to tune resolution yourself by adjusting T in a 3D-classification with a single class. Auto-refine: Angular step= 7.5 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 9.97029 Angstroms, which requires orientationSampling of at least 8 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 3528 OrientationalSampling= 15 NrOrientations= 168 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 112896 OrientationalSampling= 7.5 NrOrientations= 1344 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 3 000/??? sec ~~(,_,"> [oo] 1/ 6 sec .........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 2/ 4 sec ............................~~(,_,"> 2/ 3 sec ...............................~~(,_,"> 2/ 3 sec ..................................~~(,_,"> 2/ 3 sec .....................................~~(,_,"> 2/ 2 sec ........................................~~(,_,"> 2/ 2 sec ...........................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 3/ 3 sec .......................................................~~(,_,"> 3/ 3 sec ...........................................................~~(,_,"> 3/ 2 sec ..............................................................~~(,_,"> 3/ 3 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 8/ 8 sec ............................................................~~(,_,"> Auto-refine: Iteration= 4 Auto-refine: Resolution= 8.21082 (no gain for 0 iter) Auto-refine: Changes in angles= 15.5526 degrees; and in offsets= 0.820444 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ...................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec ......................................~~(,_,"> 0/ 0 sec .......................................~~(,_,"> 0/ 0 sec .......................................~~(,_,"> 0/ 0 sec ........................................~~(,_,"> 0/ 0 sec ........................................~~(,_,"> 0/ 0 sec .........................................~~(,_,"> 0/ 0 sec ..........................................~~(,_,"> 0/ 0 sec ..........................................~~(,_,"> 0/ 0 sec ...........................................~~(,_,"> 0/ 0 sec ...........................................~~(,_,"> 0/ 0 sec ............................................~~(,_,"> 0/ 0 sec .............................................~~(,_,"> 0/ 0 sec .............................................~~(,_,"> 0/ 0 sec ..............................................~~(,_,"> 0/ 0 sec ..............................................~~(,_,"> 0/ 0 sec ...............................................~~(,_,"> 0/ 0 sec ................................................~~(,_,"> 0/ 0 sec ................................................~~(,_,"> 0/ 0 sec .................................................~~(,_,"> 0/ 0 sec .................................................~~(,_,"> 0/ 0 sec ..................................................~~(,_,"> 0/ 0 sec ...................................................~~(,_,"> 0/ 0 sec ...................................................~~(,_,"> 0/ 0 sec ....................................................~~(,_,"> 0/ 0 sec ....................................................~~(,_,"> 0/ 0 sec .....................................................~~(,_,"> 0/ 0 sec ......................................................~~(,_,"> 0/ 0 sec ......................................................~~(,_,"> 0/ 0 sec .......................................................~~(,_,"> 0/ 0 sec .......................................................~~(,_,"> 0/ 0 sec ........................................................~~(,_,"> 0/ 0 sec .........................................................~~(,_,"> 0/ 0 sec .........................................................~~(,_,"> 0/ 0 sec ..........................................................~~(,_,"> 0/ 0 sec ..........................................................~~(,_,"> 0/ 0 sec ...........................................................~~(,_,"> 0/ 0 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 10.71 degrees; offsets= 2.04205 Angstroms Auto-refine: WARNING: The angular accuracy is worse than 10 degrees, so basically you cannot align your particles (yet)! Auto-refine: WARNING: You probably need not worry if the accuracy improves during the next few iterations. Auto-refine: WARNING: However, if the problem persists it may lead to spurious FSC curves, so be wary of inflated resolution estimates... Auto-refine: WARNING: Sometimes it is better to tune resolution yourself by adjusting T in a 3D-classification with a single class. Auto-refine: Angular step= 7.5 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 8.21082 Angstroms, which requires orientationSampling of at least 6.66667 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 3528 OrientationalSampling= 15 NrOrientations= 168 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 112896 OrientationalSampling= 7.5 NrOrientations= 1344 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 4 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 8/ 8 sec ............................................................~~(,_,"> Auto-refine: Iteration= 5 Auto-refine: Resolution= 7.34653 (no gain for 0 iter) Auto-refine: Changes in angles= 11.8146 degrees; and in offsets= 0.551989 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ...................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec ......................................~~(,_,"> 0/ 0 sec .......................................~~(,_,"> 0/ 0 sec .......................................~~(,_,"> 0/ 0 sec ........................................~~(,_,"> 0/ 0 sec ........................................~~(,_,"> 0/ 0 sec .........................................~~(,_,"> 0/ 0 sec ..........................................~~(,_,"> 0/ 0 sec ..........................................~~(,_,"> 0/ 0 sec ...........................................~~(,_,"> 0/ 0 sec ...........................................~~(,_,"> 0/ 0 sec ............................................~~(,_,"> 0/ 0 sec .............................................~~(,_,"> 0/ 0 sec .............................................~~(,_,"> 0/ 0 sec ..............................................~~(,_,"> 0/ 0 sec ..............................................~~(,_,"> 0/ 0 sec ...............................................~~(,_,"> 0/ 0 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 4.275 degrees; offsets= 1.51859 Angstroms Auto-refine: Angular step= 7.5 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 7.34653 Angstroms, which requires orientationSampling of at least 6 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 3528 OrientationalSampling= 15 NrOrientations= 168 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 112896 OrientationalSampling= 7.5 NrOrientations= 1344 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 5 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 10/ 10 sec ............................................................~~(,_,"> Auto-refine: Iteration= 6 Auto-refine: Resolution= 7.34653 (no gain for 1 iter) Auto-refine: Changes in angles= 7.16447 degrees; and in offsets= 0.504888 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 1/ 25 sec ..~~(,_,"> 1/ 20 sec ...~~(,_,"> 1/ 16 sec ...~~(,_,"> 1/ 14 sec ....~~(,_,"> 1/ 12 sec ....~~(,_,"> 1/ 11 sec .....~~(,_,"> 1/ 10 sec ......~~(,_,"> 1/ 9 sec ......~~(,_,"> 1/ 8 sec .......~~(,_,"> 1/ 7 sec .......~~(,_,"> 1/ 7 sec ........~~(,_,"> 1/ 6 sec ........~~(,_,"> 1/ 6 sec .........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec ..............~~(,_,"> 1/ 4 sec ...............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec .................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 2 sec ....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec ..........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 2/ 2 sec ........................................~~(,_,"> 2/ 2 sec ........................................~~(,_,"> 2/ 2 sec .........................................~~(,_,"> 2/ 2 sec ..........................................~~(,_,"> 2/ 2 sec ..........................................~~(,_,"> 2/ 2 sec ...........................................~~(,_,"> 2/ 2 sec ...........................................~~(,_,"> 2/ 2 sec ............................................~~(,_,"> 2/ 2 sec .............................................~~(,_,"> 2/ 2 sec .............................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec ...............................................~~(,_,"> 2/ 2 sec ................................................~~(,_,"> 2/ 2 sec ................................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec ..................................................~~(,_,"> 2/ 2 sec ...................................................~~(,_,"> 2/ 2 sec ...................................................~~(,_,"> 2/ 2 sec ....................................................~~(,_,"> 2/ 2 sec ....................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ......................................................~~(,_,"> 2/ 2 sec ......................................................~~(,_,"> 2/ 2 sec .......................................................~~(,_,"> 2/ 2 sec .......................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec .........................................................~~(,_,"> 2/ 2 sec .........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 3.355 degrees; offsets= 1.38499 Angstroms Auto-refine: Angular step= 7.5 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 7.34653 Angstroms, which requires orientationSampling of at least 6 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 3528 OrientationalSampling= 15 NrOrientations= 168 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 112896 OrientationalSampling= 7.5 NrOrientations= 1344 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 6 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 15/ 15 sec ............................................................~~(,_,"> Auto-refine: Iteration= 7 Auto-refine: Resolution= 7.34653 (no gain for 2 iter) Auto-refine: Changes in angles= 6.90086 degrees; and in offsets= 0.550648 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ...................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 3.05 degrees; offsets= 1.373 Angstroms Auto-refine: Angular step= 7.5 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 7.34653 Angstroms, which requires orientationSampling of at least 6 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 3528 OrientationalSampling= 15 NrOrientations= 168 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 112896 OrientationalSampling= 7.5 NrOrientations= 1344 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 7 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 15/ 15 sec ............................................................~~(,_,"> Auto-refine: Iteration= 8 Auto-refine: Resolution= 7.34653 (no gain for 3 iter) Auto-refine: Changes in angles= 6.39497 degrees; and in offsets= 0.593545 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 3.11 degrees; offsets= 1.33919 Angstroms Auto-refine: Angular step= 7.5 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 7.34653 Angstroms, which requires orientationSampling of at least 6 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 3528 OrientationalSampling= 15 NrOrientations= 168 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 112896 OrientationalSampling= 7.5 NrOrientations= 1344 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 8 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 14/ 14 sec ............................................................~~(,_,"> Auto-refine: Iteration= 9 Auto-refine: Resolution= 7.34653 (no gain for 4 iter) Auto-refine: Changes in angles= 7.83847 degrees; and in offsets= 0.655578 Angstroms (no gain for 1 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 3.105 degrees; offsets= 1.35828 Angstroms Auto-refine: Angular step= 3.75 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 7.34653 Angstroms, which requires orientationSampling of at least 6 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 32256 OrientationalSampling= 7.5 NrOrientations= 1536 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 1032192 OrientationalSampling= 3.75 NrOrientations= 12288 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 9 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 2/ 2 sec ...........................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec ....................................................~~(,_,"> 2/ 2 sec .......................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 3/ 2 sec ..............................................................~~(,_,"> 3/ 3 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 15/ 15 sec ............................................................~~(,_,"> Auto-refine: Iteration= 10 Auto-refine: Resolution= 5.816 (no gain for 0 iter) Auto-refine: Changes in angles= 5.79623 degrees; and in offsets= 0.537024 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 1.442 degrees; offsets= 0.801551 Angstroms Auto-refine: Angular step= 3.75 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 5.816 Angstroms, which requires orientationSampling of at least 4.73684 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 32256 OrientationalSampling= 7.5 NrOrientations= 1536 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 1032192 OrientationalSampling= 3.75 NrOrientations= 12288 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 10 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 31/ 31 sec ............................................................~~(,_,"> Auto-refine: Iteration= 11 Auto-refine: Resolution= 6.06887 (no gain for 1 iter) Auto-refine: Changes in angles= 2.75146 degrees; and in offsets= 0.40554 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec ..........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 1.502 degrees; offsets= 0.822272 Angstroms Auto-refine: Angular step= 3.75 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 6.06887 Angstroms, which requires orientationSampling of at least 4.93151 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 32256 OrientationalSampling= 7.5 NrOrientations= 1536 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 1032192 OrientationalSampling= 3.75 NrOrientations= 12288 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 11 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 31/ 31 sec ............................................................~~(,_,"> Auto-refine: Iteration= 12 Auto-refine: Resolution= 5.816 (no gain for 0 iter) Auto-refine: Changes in angles= 3.2557 degrees; and in offsets= 0.43079 Angstroms (no gain for 1 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 1/ 9 sec ......~~(,_,"> 1/ 8 sec .......~~(,_,"> 1/ 7 sec .......~~(,_,"> 1/ 7 sec ........~~(,_,"> 1/ 6 sec ........~~(,_,"> 1/ 6 sec .........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec ..............~~(,_,"> 1/ 4 sec ...............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec .................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 2 sec ....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec ..........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 2/ 2 sec ................................................~~(,_,"> 2/ 2 sec ................................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec ..................................................~~(,_,"> 2/ 2 sec ...................................................~~(,_,"> 2/ 2 sec ...................................................~~(,_,"> 2/ 2 sec ....................................................~~(,_,"> 2/ 2 sec ....................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ......................................................~~(,_,"> 2/ 2 sec ......................................................~~(,_,"> 2/ 2 sec .......................................................~~(,_,"> 2/ 2 sec .......................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec .........................................................~~(,_,"> 2/ 2 sec .........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 1.498 degrees; offsets= 0.811366 Angstroms Auto-refine: Angular step= 3.75 degrees; local searches= false Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 5.816 Angstroms, which requires orientationSampling of at least 4.73684 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 32256 OrientationalSampling= 7.5 NrOrientations= 1536 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 1032192 OrientationalSampling= 3.75 NrOrientations= 12288 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 12 000/??? sec ~~(,_,"> [oo] 1/ 6 sec .........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 2/ 2 sec ........................................~~(,_,"> 2/ 2 sec ...........................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 31/ 31 sec ............................................................~~(,_,"> Auto-refine: Iteration= 13 Auto-refine: Resolution= 6.06887 (no gain for 1 iter) Auto-refine: Changes in angles= 3.13146 degrees; and in offsets= 0.4284 Angstroms (no gain for 2 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ...................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 1.496 degrees; offsets= 0.805913 Angstroms Auto-refine: Angular step= 1.875 degrees; local searches= true Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 6.06887 Angstroms, which requires orientationSampling of at least 4.93151 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 2310 OrientationalSampling= 3.75 NrOrientations= 110 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 73920 OrientationalSampling= 1.875 NrOrientations= 880 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 13 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 0 sec ..............................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 31/ 31 sec ............................................................~~(,_,"> Auto-refine: Iteration= 14 Auto-refine: Resolution= 4.362 (no gain for 0 iter) Auto-refine: Changes in angles= 2.26794 degrees; and in offsets= 0.363263 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 1/ 3 sec .................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 2 sec ....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec ..........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.936 degrees; offsets= 0.55945 Angstroms Auto-refine: Angular step= 1.875 degrees; local searches= true Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 4.362 Angstroms, which requires orientationSampling of at least 3.56436 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 3045 OrientationalSampling= 3.75 NrOrientations= 145 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 97440 OrientationalSampling= 1.875 NrOrientations= 1160 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 14 000/??? sec ~~(,_,"> [oo] 1/ 6 sec .........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 2/ 2 sec ........................................~~(,_,"> 2/ 2 sec ...........................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 3/ 3 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 32/ 32 sec ............................................................~~(,_,"> Auto-refine: Iteration= 15 Auto-refine: Resolution= 4.362 (no gain for 1 iter) Auto-refine: Changes in angles= 1.4834 degrees; and in offsets= 0.319584 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 1/ 8 sec .......~~(,_,"> 1/ 7 sec .......~~(,_,"> 1/ 7 sec ........~~(,_,"> 1/ 6 sec ........~~(,_,"> 1/ 6 sec .........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec ..............~~(,_,"> 1/ 4 sec ...............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec .................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 2 sec ....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec ..........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 2/ 2 sec ................................................~~(,_,"> 2/ 2 sec ................................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec ..................................................~~(,_,"> 2/ 2 sec ...................................................~~(,_,"> 2/ 2 sec ...................................................~~(,_,"> 2/ 2 sec ....................................................~~(,_,"> 2/ 2 sec ....................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ......................................................~~(,_,"> 2/ 2 sec ......................................................~~(,_,"> 2/ 2 sec .......................................................~~(,_,"> 2/ 2 sec .......................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec .........................................................~~(,_,"> 2/ 2 sec .........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.931 degrees; offsets= 0.558905 Angstroms Auto-refine: Angular step= 1.875 degrees; local searches= true Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 4.362 Angstroms, which requires orientationSampling of at least 3.56436 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 2520 OrientationalSampling= 3.75 NrOrientations= 120 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 80640 OrientationalSampling= 1.875 NrOrientations= 960 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 15 000/??? sec ~~(,_,"> [oo] 1/ 6 sec .........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 2/ 3 sec ..................................~~(,_,"> 2/ 3 sec .....................................~~(,_,"> 2/ 2 sec ........................................~~(,_,"> 2/ 2 sec ...........................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 3/ 2 sec ..............................................................~~(,_,"> 3/ 3 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 33/ 33 sec ............................................................~~(,_,"> Auto-refine: Iteration= 16 Auto-refine: Resolution= 4.10541 (no gain for 0 iter) Auto-refine: Changes in angles= 1.50264 degrees; and in offsets= 0.283483 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.873 degrees; offsets= 0.534368 Angstroms Auto-refine: Angular step= 1.875 degrees; local searches= true Auto-refine: Offset search range= 2.72625 pixels; offset step= 0.54525 Anstroms CurrentResolution= 4.10541 Angstroms, which requires orientationSampling of at least 3.33333 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 2940 OrientationalSampling= 3.75 NrOrientations= 140 TranslationalSampling= 1.0905 NrTranslations= 21 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 94080 OrientationalSampling= 1.875 NrOrientations= 1120 TranslationalSampling= 0.54525 NrTranslations= 84 ============================= Expectation iteration 16 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 38/ 38 sec ............................................................~~(,_,"> Auto-refine: Iteration= 17 Auto-refine: Resolution= 4.362 (no gain for 1 iter) Auto-refine: Changes in angles= 1.60193 degrees; and in offsets= 0.311297 Angstroms (no gain for 1 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 1/ 4 sec ..............~~(,_,"> 1/ 4 sec ...............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec .................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 2 sec ....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec ..........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec .........................................................~~(,_,"> 2/ 2 sec .........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.875 degrees; offsets= 0.533822 Angstroms Auto-refine: Angular step= 0.9375 degrees; local searches= true Auto-refine: Offset search range= 1.55648 pixels; offset step= 0.400367 Anstroms CurrentResolution= 4.362 Angstroms, which requires orientationSampling of at least 3.56436 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 1350 OrientationalSampling= 1.875 NrOrientations= 150 TranslationalSampling= 0.800733 NrTranslations= 9 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 43200 OrientationalSampling= 0.9375 NrOrientations= 1200 TranslationalSampling= 0.400367 NrTranslations= 36 ============================= Expectation iteration 17 000/??? sec ~~(,_,"> [oo] 0/ 0 sec .........~~(,_,"> 0/ 0 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 2/ 2 sec ........................................~~(,_,"> 2/ 2 sec ...........................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 1 sec ..............................................................~~(,_,"> 3/ 3 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 32/ 32 sec ............................................................~~(,_,"> Auto-refine: Iteration= 18 Auto-refine: Resolution= 3.87733 (no gain for 0 iter) Auto-refine: Changes in angles= 1.17649 degrees; and in offsets= 0.259622 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 1/ 9 sec ......~~(,_,"> 1/ 8 sec .......~~(,_,"> 1/ 7 sec .......~~(,_,"> 1/ 7 sec ........~~(,_,"> 1/ 6 sec ........~~(,_,"> 1/ 6 sec .........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec ..............~~(,_,"> 1/ 4 sec ...............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec .................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 2 sec ....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec ..........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 2/ 2 sec ....................................................~~(,_,"> 2/ 2 sec .....................................................~~(,_,"> 2/ 2 sec ......................................................~~(,_,"> 2/ 2 sec ......................................................~~(,_,"> 2/ 2 sec .......................................................~~(,_,"> 2/ 2 sec .......................................................~~(,_,"> 2/ 2 sec ........................................................~~(,_,"> 2/ 2 sec .........................................................~~(,_,"> 2/ 2 sec .........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ..........................................................~~(,_,"> 2/ 2 sec ...........................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.672 degrees; offsets= 0.430766 Angstroms Auto-refine: Angular step= 0.9375 degrees; local searches= true Auto-refine: Offset search range= 1.55648 pixels; offset step= 0.400367 Anstroms CurrentResolution= 3.87733 Angstroms, which requires orientationSampling of at least 3.15789 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 1305 OrientationalSampling= 1.875 NrOrientations= 145 TranslationalSampling= 0.800733 NrTranslations= 9 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 41760 OrientationalSampling= 0.9375 NrOrientations= 1160 TranslationalSampling= 0.400367 NrTranslations= 36 ============================= Expectation iteration 18 000/??? sec ~~(,_,"> [oo] 1/ 6 sec .........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 2/ 4 sec ............................~~(,_,"> 2/ 3 sec ...............................~~(,_,"> 2/ 3 sec ..................................~~(,_,"> 2/ 3 sec .....................................~~(,_,"> 2/ 2 sec ........................................~~(,_,"> 2/ 2 sec ...........................................~~(,_,"> 2/ 2 sec ..............................................~~(,_,"> 2/ 2 sec .................................................~~(,_,"> 3/ 3 sec .....................................................~~(,_,"> 3/ 3 sec ........................................................~~(,_,"> 3/ 3 sec ...........................................................~~(,_,"> 3/ 2 sec ..............................................................~~(,_,"> 3/ 3 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 20/ 20 sec ............................................................~~(,_,"> Auto-refine: Iteration= 19 Auto-refine: Resolution= 3.87733 (no gain for 1 iter) Auto-refine: Changes in angles= 0.593377 degrees; and in offsets= 0.197395 Angstroms (no gain for 1 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ...................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.673 degrees; offsets= 0.425858 Angstroms Auto-refine: Angular step= 0.46875 degrees; local searches= true Auto-refine: Offset search range= 0.986976 pixels; offset step= 0.319394 Anstroms CurrentResolution= 3.87733 Angstroms, which requires orientationSampling of at least 3.15789 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 1305 OrientationalSampling= 0.9375 NrOrientations= 145 TranslationalSampling= 0.638787 NrTranslations= 9 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 41760 OrientationalSampling= 0.46875 NrOrientations= 1160 TranslationalSampling= 0.319394 NrTranslations= 36 ============================= Expectation iteration 19 000/??? sec ~~(,_,"> [oo] 1/ 6 sec .........~~(,_,"> 1/ 4 sec ............~~(,_,"> 2/ 7 sec ...............~~(,_,"> 2/ 6 sec ..................~~(,_,"> 2/ 5 sec .....................~~(,_,"> 2/ 4 sec ........................~~(,_,"> 3/ 6 sec ............................~~(,_,"> 3/ 5 sec ...............................~~(,_,"> 3/ 5 sec ..................................~~(,_,"> 3/ 4 sec .....................................~~(,_,"> 4/ 5 sec ........................................~~(,_,"> 4/ 5 sec ...........................................~~(,_,"> 4/ 5 sec ..............................................~~(,_,"> 4/ 4 sec .................................................~~(,_,"> 5/ 5 sec .....................................................~~(,_,"> 5/ 5 sec ........................................................~~(,_,"> 5/ 5 sec ...........................................................~~(,_,"> 6/ 5 sec ..............................................................~~(,_,"> 6/ 6 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 20/ 20 sec ............................................................~~(,_,"> Auto-refine: Iteration= 20 Auto-refine: Resolution= 3.77254 (no gain for 0 iter) Auto-refine: Changes in angles= 0.550745 degrees; and in offsets= 0.169753 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ...................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec .....................................~~(,_,"> 0/ 0 sec ......................................~~(,_,"> 0/ 0 sec .......................................~~(,_,"> 0/ 0 sec .......................................~~(,_,"> 0/ 0 sec ........................................~~(,_,"> 0/ 0 sec ........................................~~(,_,"> 0/ 0 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.595 degrees; offsets= 0.381691 Angstroms Auto-refine: Angular step= 0.46875 degrees; local searches= true Auto-refine: Offset search range= 0.986976 pixels; offset step= 0.319394 Anstroms CurrentResolution= 3.77254 Angstroms, which requires orientationSampling of at least 3.07692 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 1260 OrientationalSampling= 0.9375 NrOrientations= 140 TranslationalSampling= 0.638787 NrTranslations= 9 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 40320 OrientationalSampling= 0.46875 NrOrientations= 1120 TranslationalSampling= 0.319394 NrTranslations= 36 ============================= Expectation iteration 20 000/??? sec ~~(,_,"> [oo] 1/ 6 sec .........~~(,_,"> 1/ 4 sec ............~~(,_,"> 2/ 7 sec ...............~~(,_,"> 2/ 6 sec ..................~~(,_,"> 2/ 5 sec .....................~~(,_,"> 2/ 4 sec ........................~~(,_,"> 3/ 6 sec ............................~~(,_,"> 3/ 5 sec ...............................~~(,_,"> 3/ 5 sec ..................................~~(,_,"> 3/ 4 sec .....................................~~(,_,"> 4/ 5 sec ........................................~~(,_,"> 4/ 5 sec ...........................................~~(,_,"> 4/ 5 sec ..............................................~~(,_,"> 5/ 6 sec .................................................~~(,_,"> 5/ 5 sec .....................................................~~(,_,"> 5/ 5 sec ........................................................~~(,_,"> 6/ 6 sec ...........................................................~~(,_,"> 6/ 5 sec ..............................................................~~(,_,"> 6/ 6 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 27/ 27 sec ............................................................~~(,_,"> Auto-refine: Iteration= 21 Auto-refine: Resolution= 3.77254 (no gain for 1 iter) Auto-refine: Changes in angles= 0.36543 degrees; and in offsets= 0.133595 Angstroms (no gain for 1 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 1/ 2 sec ....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec ..........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.586 degrees; offsets= 0.381691 Angstroms Auto-refine: Angular step= 0.234375 degrees; local searches= true Auto-refine: Offset search range= 0.858805 pixels; offset step= 0.286268 Anstroms CurrentResolution= 3.77254 Angstroms, which requires orientationSampling of at least 3.07692 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 1305 OrientationalSampling= 0.46875 NrOrientations= 145 TranslationalSampling= 0.572537 NrTranslations= 9 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 41760 OrientationalSampling= 0.234375 NrOrientations= 1160 TranslationalSampling= 0.286268 NrTranslations= 36 ============================= Expectation iteration 21 000/??? sec ~~(,_,"> [oo] 2/ 12 sec .........~~(,_,"> 2/ 9 sec ............~~(,_,"> 3/ 11 sec ...............~~(,_,"> 3/ 9 sec ..................~~(,_,"> 5/ 13 sec .....................~~(,_,"> 5/ 12 sec ........................~~(,_,"> 7/ 14 sec ............................~~(,_,"> 7/ 13 sec ...............................~~(,_,"> 8/ 13 sec ..................................~~(,_,"> 8/ 12 sec .....................................~~(,_,"> 10/ 14 sec ........................................~~(,_,"> 10/ 13 sec ...........................................~~(,_,"> 11/ 14 sec ..............................................~~(,_,"> 11/ 13 sec .................................................~~(,_,"> 13/ 14 sec .....................................................~~(,_,"> 13/ 13 sec ........................................................~~(,_,"> 14/ 14 sec ...........................................................~~(,_,"> 16/ 15 sec ..............................................................~~(,_,"> 17/ 17 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 27/ 27 sec ............................................................~~(,_,"> Auto-refine: Iteration= 22 Auto-refine: Resolution= 3.77254 (no gain for 1 iter) Auto-refine: Changes in angles= 0.228374 degrees; and in offsets= 0.129629 Angstroms (no gain for 0 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 1/ 7 sec .......~~(,_,"> 1/ 7 sec ........~~(,_,"> 1/ 6 sec ........~~(,_,"> 1/ 6 sec .........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec ..............~~(,_,"> 1/ 4 sec ...............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec .................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 2 sec ....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec ......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec .......................~~(,_,"> 1/ 2 sec ........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec .........................~~(,_,"> 1/ 2 sec ..........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ...........................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec ............................~~(,_,"> 1/ 2 sec .............................~~(,_,"> 1/ 2 sec ..............................~~(,_,"> 1/ 1 sec ..............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ...............................~~(,_,"> 1/ 1 sec ................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec .................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ..................................~~(,_,"> 1/ 1 sec ...................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 2/ 2 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.556 degrees; offsets= 0.380055 Angstroms Auto-refine: Angular step= 0.234375 degrees; local searches= true Auto-refine: Offset search range= 0.858805 pixels; offset step= 0.286268 Anstroms CurrentResolution= 3.77254 Angstroms, which requires orientationSampling of at least 3.07692 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 1395 OrientationalSampling= 0.46875 NrOrientations= 155 TranslationalSampling= 0.572537 NrTranslations= 9 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 44640 OrientationalSampling= 0.234375 NrOrientations= 1240 TranslationalSampling= 0.286268 NrTranslations= 36 ============================= Expectation iteration 22 000/??? sec ~~(,_,"> [oo] 2/ 12 sec .........~~(,_,"> 2/ 9 sec ............~~(,_,"> 4/ 15 sec ...............~~(,_,"> 4/ 12 sec ..................~~(,_,"> 5/ 13 sec .....................~~(,_,"> 5/ 12 sec ........................~~(,_,"> 7/ 14 sec ............................~~(,_,"> 7/ 13 sec ...............................~~(,_,"> 8/ 13 sec ..................................~~(,_,"> 8/ 12 sec .....................................~~(,_,"> 10/ 14 sec ........................................~~(,_,"> 10/ 13 sec ...........................................~~(,_,"> 12/ 15 sec ..............................................~~(,_,"> 12/ 14 sec .................................................~~(,_,"> 13/ 14 sec .....................................................~~(,_,"> 13/ 13 sec ........................................................~~(,_,"> 15/ 15 sec ...........................................................~~(,_,"> 16/ 15 sec ..............................................................~~(,_,"> 17/ 17 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 27/ 27 sec ............................................................~~(,_,"> Auto-refine: Iteration= 23 Auto-refine: Resolution= 3.77254 (no gain for 2 iter) Auto-refine: Changes in angles= 0.164948 degrees; and in offsets= 0.100554 Angstroms (no gain for 1 iter) Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec .......~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec ........~~(,_,"> 0/ 0 sec .........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ..........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ...........~~(,_,"> 0/ 0 sec ............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec .............~~(,_,"> 0/ 0 sec ..............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ...............~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec ................~~(,_,"> 0/ 0 sec .................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ..................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ...................~~(,_,"> 0/ 0 sec ....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec .....................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec ......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec .......................~~(,_,"> 0/ 0 sec ........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec .........................~~(,_,"> 0/ 0 sec ..........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ...........................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec ............................~~(,_,"> 0/ 0 sec .............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ..............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ...............................~~(,_,"> 0/ 0 sec ................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec .................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ..................................~~(,_,"> 0/ 0 sec ...................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 0/ 0 sec ....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec .....................................~~(,_,"> 1/ 1 sec ......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec .......................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec ........................................~~(,_,"> 1/ 1 sec .........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ..........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ...........................................~~(,_,"> 1/ 1 sec ............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec .............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ..............................................~~(,_,"> 1/ 1 sec ...............................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec ................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec .................................................~~(,_,"> 1/ 1 sec ..................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ...................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec ....................................................~~(,_,"> 1/ 1 sec .....................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec ......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec .......................................................~~(,_,"> 1/ 1 sec ........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec .........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ..........................................................~~(,_,"> 1/ 1 sec ...........................................................~~(,_,"> 1/ 1 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.564 degrees; offsets= 0.376238 Angstroms Auto-refine: Angular step= 0.234375 degrees; local searches= true Auto-refine: Offset search range= 0.858805 pixels; offset step= 0.286268 Anstroms CurrentResolution= 3.77254 Angstroms, which requires orientationSampling of at least 3.07692 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 1350 OrientationalSampling= 0.46875 NrOrientations= 150 TranslationalSampling= 0.572537 NrTranslations= 9 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 43200 OrientationalSampling= 0.234375 NrOrientations= 1200 TranslationalSampling= 0.286268 NrTranslations= 36 ============================= Expectation iteration 23 000/??? sec ~~(,_,"> [oo] 1/ 6 sec .........~~(,_,"> 1/ 4 sec ............~~(,_,"> 3/ 11 sec ...............~~(,_,"> 3/ 9 sec ..................~~(,_,"> 5/ 13 sec .....................~~(,_,"> 5/ 12 sec ........................~~(,_,"> 6/ 12 sec ............................~~(,_,"> 6/ 11 sec ...............................~~(,_,"> 8/ 13 sec ..................................~~(,_,"> 8/ 12 sec .....................................~~(,_,"> 9/ 13 sec ........................................~~(,_,"> 9/ 12 sec ...........................................~~(,_,"> 11/ 14 sec ..............................................~~(,_,"> 11/ 13 sec .................................................~~(,_,"> 13/ 14 sec .....................................................~~(,_,"> 13/ 13 sec ........................................................~~(,_,"> 14/ 14 sec ...........................................................~~(,_,"> 16/ 15 sec ..............................................................~~(,_,"> 16/ 16 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 27/ 27 sec ............................................................~~(,_,"> Auto-refine: Iteration= 24 Auto-refine: Resolution= 3.77254 (no gain for 3 iter) Auto-refine: Changes in angles= 0.144979 degrees; and in offsets= 0.145627 Angstroms (no gain for 2 iter) Auto-refine: Refinement has converged, entering last iteration where two halves will be combined... Auto-refine: The last iteration will use data to Nyquist frequency, which may take more CPU and RAM. Estimating accuracies in the orientational assignment ... 000/??? sec ~~(,_,"> [oo] 0/ 0 sec ~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec .~~(,_,"> 0/ 0 sec ..~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ...~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec ....~~(,_,"> 0/ 0 sec .....~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec ......~~(,_,"> 0/ 0 sec .......~~(,_,"> 1/ 7 sec .......~~(,_,"> 1/ 7 sec ........~~(,_,"> 1/ 6 sec ........~~(,_,"> 1/ 6 sec .........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ..........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 5 sec ...........~~(,_,"> 1/ 4 sec ............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec .............~~(,_,"> 1/ 4 sec ..............~~(,_,"> 1/ 4 sec ...............~~(,_,"> 1/ 3 sec ...............~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec ................~~(,_,"> 1/ 3 sec .................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ..................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 3 sec ...................~~(,_,"> 1/ 2 sec ....................~~(,_,"> 1/ 2 sec .....................~~(,_,"> 2/ 5 sec .....................~~(,_,"> 2/ 5 sec ......................~~(,_,"> 2/ 5 sec ......................~~(,_,"> 2/ 5 sec .......................~~(,_,"> 2/ 5 sec .......................~~(,_,"> 2/ 4 sec ........................~~(,_,"> 2/ 4 sec .........................~~(,_,"> 2/ 4 sec .........................~~(,_,"> 2/ 4 sec ..........................~~(,_,"> 2/ 4 sec ...........................~~(,_,"> 2/ 4 sec ...........................~~(,_,"> 2/ 4 sec ............................~~(,_,"> 2/ 4 sec ............................~~(,_,"> 2/ 4 sec .............................~~(,_,"> 2/ 4 sec ..............................~~(,_,"> 2/ 3 sec ..............................~~(,_,"> 2/ 3 sec ...............................~~(,_,"> 2/ 3 sec ...............................~~(,_,"> 2/ 3 sec ................................~~(,_,"> 2/ 3 sec .................................~~(,_,"> 2/ 3 sec .................................~~(,_,"> 2/ 3 sec ..................................~~(,_,"> 3/ 5 sec ..................................~~(,_,"> 3/ 5 sec ...................................~~(,_,"> 3/ 5 sec ....................................~~(,_,"> 3/ 4 sec ....................................~~(,_,"> 3/ 4 sec .....................................~~(,_,"> 3/ 4 sec .....................................~~(,_,"> 3/ 4 sec ......................................~~(,_,"> 3/ 4 sec .......................................~~(,_,"> 3/ 4 sec .......................................~~(,_,"> 3/ 4 sec ........................................~~(,_,"> 3/ 4 sec ........................................~~(,_,"> 3/ 4 sec .........................................~~(,_,"> 3/ 4 sec ..........................................~~(,_,"> 3/ 4 sec ..........................................~~(,_,"> 3/ 4 sec ...........................................~~(,_,"> 3/ 4 sec ...........................................~~(,_,"> 3/ 4 sec ............................................~~(,_,"> 3/ 4 sec .............................................~~(,_,"> 3/ 3 sec .............................................~~(,_,"> 3/ 3 sec ..............................................~~(,_,"> 3/ 3 sec ..............................................~~(,_,"> 3/ 3 sec ...............................................~~(,_,"> 3/ 3 sec ................................................~~(,_,"> 4/ 4 sec ................................................~~(,_,"> 4/ 4 sec .................................................~~(,_,"> 4/ 4 sec .................................................~~(,_,"> 4/ 4 sec ..................................................~~(,_,"> 4/ 4 sec ...................................................~~(,_,"> 4/ 4 sec ...................................................~~(,_,"> 4/ 4 sec ....................................................~~(,_,"> 4/ 4 sec ....................................................~~(,_,"> 4/ 4 sec .....................................................~~(,_,"> 4/ 4 sec ......................................................~~(,_,"> 4/ 4 sec ......................................................~~(,_,"> 4/ 4 sec .......................................................~~(,_,"> 4/ 4 sec .......................................................~~(,_,"> 4/ 4 sec ........................................................~~(,_,"> 4/ 4 sec .........................................................~~(,_,"> 4/ 4 sec .........................................................~~(,_,"> 4/ 4 sec ..........................................................~~(,_,"> 4/ 4 sec ..........................................................~~(,_,"> 4/ 4 sec ...........................................................~~(,_,"> 4/ 4 sec ............................................................~~(,_,"> Auto-refine: Estimated accuracy angles= 0.576 degrees; offsets= 0.376238 Angstroms Auto-refine: Angular step= 0.234375 degrees; local searches= true Auto-refine: Offset search range= 0.858805 pixels; offset step= 0.286268 Anstroms CurrentResolution= 3.77254 Angstroms, which requires orientationSampling of at least 3.07692 degrees for a particle of diameter 140 Angstroms Oversampling= 0 NrHiddenVariableSamplingPoints= 1350 OrientationalSampling= 0.46875 NrOrientations= 150 TranslationalSampling= 0.572537 NrTranslations= 9 ============================= Oversampling= 1 NrHiddenVariableSamplingPoints= 43200 OrientationalSampling= 0.234375 NrOrientations= 1200 TranslationalSampling= 0.286268 NrTranslations= 36 ============================= Expectation iteration 24 000/??? sec ~~(,_,"> [oo] 2/ 12 sec .........~~(,_,"> 2/ 9 sec ............~~(,_,"> 4/ 15 sec ...............~~(,_,"> 5/ 16 sec ..................~~(,_,"> 6/ 16 sec .....................~~(,_,"> 7/ 16 sec ........................~~(,_,"> 9/ 19 sec ............................~~(,_,"> 9/ 17 sec ...............................~~(,_,"> 11/ 19 sec ..................................~~(,_,"> 11/ 17 sec .....................................~~(,_,"> 13/ 19 sec ........................................~~(,_,"> 13/ 17 sec ...........................................~~(,_,"> 15/ 19 sec ..............................................~~(,_,"> 15/ 18 sec .................................................~~(,_,"> 17/ 19 sec .....................................................~~(,_,"> 17/ 18 sec ........................................................~~(,_,"> 19/ 19 sec ...........................................................~~(,_,"> 21/ 20 sec ..............................................................~~(,_,"> 22/ 22 sec ............................................................~~(,_,"> Averaging half-reconstructions up to 40 Angstrom resolution to prevent diverging orientations ... Note that only for higher resolutions the FSC-values are according to the gold-standard! Calculating gold-standard FSC ... Maximization ... 000/??? sec ~~(,_,"> [oo] 7.75/7.75 min ............................................................~~(,_,"> Auto-refine: Refinement has converged, stopping now... Auto-refine: + Final reconstruction from all particles is saved as: Refine3D/job018/run_class001.mrc Auto-refine: + Final model parameters are stored in: Refine3D/job018/run_model.star Auto-refine: + Final data parameters are stored in: Refine3D/job018/run_data.star Auto-refine: + Final resolution (without masking) is: 3.10187 Auto-refine: + But you may want to run relion_postprocess to mask the unfil.mrc maps and calculate a higher resolution FSC