>> Selection of GroEL micrographs taken on 2020/02/17 << 2020/02/21 Takeshi Kawabata ## CAUTION : This data is open during one month ! ## ## This site has to be closed on 2020/03/17 ! ## This data was taken on 2020/02/17 for GroEL molecule for the CryoEM workshop (2020/02/17--19) in IPR, Osaka Univerisity. >> Parameters << Talos 50 e/ A**2 73 kV 16 frames 1.4 angstrom/pix Cs 2.7 >> Micrographs image data << # Original image data has *.mrc format with 16 bit interger depth. The command "relion_convert_to_tiff" was used to convert *.mrc to *.tif. # All the 500 micrographs were analyzed by MotionCorr and CtfFind4 through relino 3.1. The values FigureOfMerit and MaxResolution stored in CtfFind/job002/micrographs_ctf.star were used to select the best micrographs. * movie/*.tif [178GB]: 500 micrographs movie data * movie159/*.tif [57 GB]: 159 micrographs movie data with FigureOfMerit >0.03 and MaxResolution < 25 angstrom awk '($0!~/^#/)&&(length($0)>50)&&($8>0.03)&&($9<25.0){print "cp ",substr($1,25,66)".tif ../movie159/"}' micrographs_ctf.txt > cp_movie159 * movie20/*.tif [7.1GB] : 20 best-MaxResolution micrographs movie data with FigureOfMerit >0.03 awk '($0!~/^#/)&&(length($0)>50)&&($8>0.03){print }' micrographs_ctf.txt |sort -k 9 | head -20 |awk '{print "cp ",substr($1,25,66)".tif ../movie20/"}' > cp_movie20